Nature Communications (Oct 2019)

Rapid selection and identification of functional CD8+ T cell epitopes from large peptide-coding libraries

  • Govinda Sharma,
  • Craig M. Rive,
  • Robert A. Holt

DOI
https://doi.org/10.1038/s41467-019-12444-7
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 13

Abstract

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High-throughput assays for TCR specificity are a bottleneck in understanding T cell immunity and harnessing it for medicine. Here the authors develop a functional screening method to identify T cell specificity in the natural context of peptide-MHC presentation, enabling detection of physiologically relevant T cell antigens from large libraries of peptide-coding sequences.