Nature Communications (Oct 2021)
Physiologic biomechanics enhance reproducible contractile development in a stem cell derived cardiac muscle platform
- Yao-Chang Tsan,
- Samuel J. DePalma,
- Yan-Ting Zhao,
- Adela Capilnasiu,
- Yu-Wei Wu,
- Brynn Elder,
- Isabella Panse,
- Kathryn Ufford,
- Daniel L. Matera,
- Sabrina Friedline,
- Thomas S. O’Leary,
- Nadab Wubshet,
- Kenneth K. Y. Ho,
- Michael J. Previs,
- David Nordsletten,
- Lori L. Isom,
- Brendon M. Baker,
- Allen P. Liu,
- Adam S. Helms
Affiliations
- Yao-Chang Tsan
- Division of Cardiovascular Medicine, University of Michigan
- Samuel J. DePalma
- Department of Biomedical Engineering, University of Michigan
- Yan-Ting Zhao
- Department of Pharmacology, University of Michigan
- Adela Capilnasiu
- Department of Biomedical Engineering, University of Michigan
- Yu-Wei Wu
- Institute of Molecular Biology, Academia Sinica
- Brynn Elder
- Division of Cardiovascular Medicine, University of Michigan
- Isabella Panse
- Division of Cardiovascular Medicine, University of Michigan
- Kathryn Ufford
- Division of Cardiovascular Medicine, University of Michigan
- Daniel L. Matera
- Department of Biomedical Engineering, University of Michigan
- Sabrina Friedline
- Division of Cardiovascular Medicine, University of Michigan
- Thomas S. O’Leary
- Molecular Physiology and Biophysics, University of Vermont
- Nadab Wubshet
- Department of Mechanical Engineering, University of Michigan
- Kenneth K. Y. Ho
- Department of Mechanical Engineering, University of Michigan
- Michael J. Previs
- Molecular Physiology and Biophysics, University of Vermont
- David Nordsletten
- Department of Biomedical Engineering, University of Michigan
- Lori L. Isom
- Department of Pharmacology, University of Michigan
- Brendon M. Baker
- Department of Biomedical Engineering, University of Michigan
- Allen P. Liu
- Department of Biomedical Engineering, University of Michigan
- Adam S. Helms
- Division of Cardiovascular Medicine, University of Michigan
- DOI
- https://doi.org/10.1038/s41467-021-26496-1
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 16
Abstract
Investigations of human cardiac disease involving human pluripotent stem cell-derived cardiomyocytes are limited by the disorganized presentation of biomechanical cues resulting in cell immaturity. Here the authors develop a platform of micron-scale 2D cardiac muscle bundles to precisely deliver physiologic cues, improving reproducibility and throughput.
