Biology (Dec 2021)

Replication of Integrative Data Analysis for Adipose Tissue Dysfunction, Low-Grade Inflammation, Postprandial Responses and OMICs Signatures in Symptom-Free Adults

  • Esther C. Gallegos-Cabriales,
  • Ernesto Rodriguez-Ayala,
  • Hugo A. Laviada-Molina,
  • Edna J. Nava-Gonzalez,
  • Rocío A. Salinas-Osornio,
  • Lorena Orozco,
  • Irene Leal-Berumen,
  • Juan Carlos Castillo-Pineda,
  • Laura Gonzalez-Lopez,
  • Claudia Escudero-Lourdes,
  • Judith Cornejo-Barrera,
  • Fabiola Escalante-Araiza,
  • Eira E. Huerta-Avila,
  • Fatima A. Buenfil-Rello,
  • Vanessa-Giselle Peschard,
  • Eliud Silva,
  • Rosa A. Veloz-Garza,
  • Angelica Martinez-Hernandez,
  • Francisco M. Barajas-Olmos,
  • Fernanda Molina-Segui,
  • Lucia Gonzalez-Ramirez,
  • Ruy D. Arjona-Villicaña,
  • Victor M. Hernandez-Escalante,
  • Janeth F. Gaytan-Saucedo,
  • Zoila Vaquera,
  • Monica Acebo-Martinez,
  • Areli Murillo-Ramirez,
  • Sara P. Diaz-Tena,
  • Benigno Figueroa-Nuñez,
  • Melesio E. Valencia-Rendon,
  • Rafael Garzon-Zamora,
  • Juan Manuel Viveros-Paredes,
  • Salvador B. Valdovinos-Chavez,
  • Anthony G Comuzzie,
  • Karin Haack,
  • Ashley A. Thorsell,
  • Xianlin Han,
  • Shelley A. Cole,
  • Raul A. Bastarrachea

DOI
https://doi.org/10.3390/biology10121342
Journal volume & issue
Vol. 10, no. 12
p. 1342

Abstract

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We previously reported preliminary characterization of adipose tissue (AT) dysfunction through the adiponectin/leptin ratio (ALR) and fasting/postprandial (F/P) gene expression in subcutaneous (SQ) adipose tissue (AT) biopsies obtained from participants in the GEMM study, a precision medicine research project. Here we present integrative data replication of previous findings from an increased number of GEMM symptom-free (SF) adults (N = 124) to improve characterization of early biomarkers for cardiovascular (CV)/immunometabolic risk in SF adults with AT dysfunction. We achieved this goal by taking advantage of the rich set of GEMM F/P 5 h time course data and three tissue samples collected at the same time and frequency on each adult participant (F/P blood, biopsies of SQAT and skeletal muscle (SKM)). We classified them with the presence/absence of AT dysfunction: low (1) ALR. We also examined the presence of metabolically healthy (MH)/unhealthy (MUH) individuals through low-grade chronic subclinical inflammation (high sensitivity C-reactive protein (hsCRP)), whole body insulin sensitivity (Matsuda Index) and Metabolic Syndrome criteria in people with/without AT dysfunction. Molecular data directly measured from three tissues in a subset of participants allowed fine-scale multi-OMIC profiling of individual postprandial responses (RNA-seq in SKM and SQAT, miRNA from plasma exosomes and shotgun lipidomics in blood). Dynamic postprandial immunometabolic molecular endophenotypes were obtained to move towards a personalized, patient-defined medicine. This study offers an example of integrative translational research, which applies bench-to-bedside research to clinical medicine. Our F/P study design has the potential to characterize CV/immunometabolic early risk detection in support of precision medicine and discovery in SF individuals.

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