Cell Journal (Jan 2010)
The Effects of a Single Dosage of Diazinon and Hinosan on the Structure of Testis Tissue and Sexual Hormones in Mice
Abstract
Objective: Diazinon (DZN) and Hinosan, two of the most important organophosphatepesticides, are widely used for pest control in gardens and agriculture. These compoundsadversely effect enzyme activation and reproductive organs. Therefore, in the presentstudy, we investigated DZN and Hinosan impacts on the structure of testis tissue andsexual hormones in mice.Materials and Methods: For this study, 60 male Balb/C mice were divided into the followinggroups: DZN, Hinosan, control and sham. In the experimental groups, mice wereinjected with a single dose of DZN (30 mg/kg intraperitoneal) and Hinosan (20 mg/kgintraperitoneal), sham (corn oil) and control (no injection). Animals were sacrificed 35days after the latest injection. Blood samples were collected and testosterone, luteinzinghormones (LH) and Follicle stimulating hormone (FSH) levels were assayed. Testistissues sections were provided to investigate the changes of spermatogenic and Leydigcells. Testis and seminiferous diameter were assayed with micrometer and eye piece,respectively. Data were analyzed with one-way ANOVA. Significance was set at p<0.05.Results: In the present study, no significant differences in testis and seminiferous diameterand number of blood vessels were noted. However the number of germ cells,spermatocysts, spermatids and Leydig cells on the testes of mice decreased (p<0.05).There was no significant difference between the parameters of the sham group comparedto the control group.Conclusion: These results suggest that DZN and Hinosan can exert a decrease in spermproduction. Therefore, application of DZN should be limited to a designed program.
