Total serum pentosidine quantification using liquid chromatography-tandem mass spectrometry
Lindsie A. Blencowe,
Andrea Božović,
Evelyn Wong,
Vathany Kulasingam,
Angela M. Cheung
Affiliations
Lindsie A. Blencowe
Institute of Medical Science, University of Toronto, Toronto, ON, Canada; Toronto General Hospital Research Institute, University Health Network, Toronto, ON, Canada
Andrea Božović
Department of Clinical Biochemistry, University Health Network, Toronto, ON, Canada; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada
Evelyn Wong
Department of Medicine, University Health Network, Toronto, ON, Canada
Vathany Kulasingam
Department of Clinical Biochemistry, University Health Network, Toronto, ON, Canada; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada
Angela M. Cheung
Institute of Medical Science, University of Toronto, Toronto, ON, Canada; Toronto General Hospital Research Institute, University Health Network, Toronto, ON, Canada; Department of Medicine, University Health Network, Toronto, ON, Canada; Corresponding author at: 200 Elizabeth Street, 7EN-221A, Toronto, ON M5G 2C4, Canada.
Pentosidine (PEN) is an Advanced Glycation End-product (AGE) that is known to accumulate in bone collagen with aging and contribute to fracture risk. The PEN content in bone is correlated with serum PEN, making it an attractive, potential osteoporosis biomarker. We sought to develop a method for quantifying PEN in stored serum. After conducting a systematic narrative review of PEN quantification methodologies, we developed a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for quantifying total serum PEN. Our method is both sensitive and precise (LOD 2 nM, LOQ 5 nM, %CV < 6.5 % and recovery 91.2–100.7 %). Our method is also equivalent or better than other methods identified in our review. Additionally, LC-MS/MS avoids the pitfalls and limitations of using fluorescence as a means of detection and could be adapted to investigate a broad range of AGE compounds.
