Turbidimetric Method for Standardization and Quality Control of Aminoglycoside Antibiotics and Aminoglycoside-Based Products

Abstract

The article covers microbiological methods that help assess the biological activity of antibiotics, which is fundamental for determination of clinical efficacy of antimicrobial drugs. The authors chose gentamicin, an aminoglycoside antibiotic, to illustrate the development and validation of the turbidimetric assay, which is based on antibiotic's inhibitory effect on the growth of Staphylococcus aureus ATCC 6538Р test strain in liquid medium. The obtained data were assessed with ANOVA and were recognized as linear (r=0.9921) in the concentration range of 0.64-1.56 mg/ml. The results of gentamicin assay validation studies demonstrate great potential and applicability of the turbidimetric method for standardization and ensuing quality control of aminoglycoside antibiotics.

Keywords

• aminoglycoside antibiotic
• assay
• turbidimetric method
• standardization
• quality control