Quantitative Trait Loci Mapping for Adult-Plant Stripe Rust Resistance in Chinese Wheat Cultivar Weimai 8

Xiaocui Yan; Xiaoling Zhang; Mengyun Kou; Takele Weldu Gebrewahid; Jiaxin Xi; Zaifeng Li; Zhanjun Yao

doi:10.3390/agronomy14020264

Agronomy (Jan 2024)

Quantitative Trait Loci Mapping for Adult-Plant Stripe Rust Resistance in Chinese Wheat Cultivar Weimai 8

Xiaocui Yan,
Xiaoling Zhang,
Mengyun Kou,
Takele Weldu Gebrewahid,
Jiaxin Xi,
Zaifeng Li,
Zhanjun Yao

Affiliations

Xiaocui Yan: North China Key Laboratory for Crop Germplasm Resources of China’s Ministry of Education, College of Agronomy, Hebei Agricultural University, Baoding 071001, China
Xiaoling Zhang: North China Key Laboratory for Crop Germplasm Resources of China’s Ministry of Education, College of Agronomy, Hebei Agricultural University, Baoding 071001, China
Mengyun Kou: North China Key Laboratory for Crop Germplasm Resources of China’s Ministry of Education, College of Agronomy, Hebei Agricultural University, Baoding 071001, China
Takele Weldu Gebrewahid: North China Key Laboratory for Crop Germplasm Resources of China’s Ministry of Education, College of Agronomy, Hebei Agricultural University, Baoding 071001, China
Jiaxin Xi: North China Key Laboratory for Crop Germplasm Resources of China’s Ministry of Education, College of Agronomy, Hebei Agricultural University, Baoding 071001, China
Zaifeng Li: College of Plant Protection, Hebei Agricultural University, Baoding 071001, China
Zhanjun Yao: North China Key Laboratory for Crop Germplasm Resources of China’s Ministry of Education, College of Agronomy, Hebei Agricultural University, Baoding 071001, China

DOI: https://doi.org/10.3390/agronomy14020264
Journal volume & issue: Vol. 14, no. 2
p. 264

Abstract

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Wheat stripe rust, triggered by Puccinia striiformis f. sp. tritici, is among the most widespread and damaging wheat (Triticum L.) diseases. The development of cultivars harboring adult plant resistance (APR) to stripe rust is a better approach to control the disease. The current study aimed to map APR to stripe rust via the QTL mapping of 165 F2–6 recombinant inbred lines (RILs), derivatives of Weimai 8/Zhengzhou 5389. The collection of phenotypic data for the stripe-rust resistance of both parents and all 165 RILs were conducted at Baoding, Hebei Province, during the 2016–2017 cropping seasons, and at Mianyang, Sichuan Province, during the 2017–2018 and 2018–2019 sowing seasons. The RIL populations and parents were also genotyped with 860 pairs of simple-sequence-repeat (SSR) primers to map APR QTLs to stripe-rust resistant. Moreover, a 55K SNP chip was used for small group bulk segregant analysis conducted to locate the genetic map location and concentration of the SNP markers on the wheat genome. Inclusive composite interval mapping (IciMapping 3.2) software identified four QTLs of stripe-rust resistance on chromosomes 1B, 2AS, 2BS, and 7DS, named QYr.wmy-1B, QYr.wmy-2AS, QYr.wmy-2BS, and QYr.wmy-7DS, which significantly explained 11.56–16.64%, 9.35–12.70%, 7.27–9.95%, and 11.49–15.07% of the phenotypic variation, respectively. All these QTLs were found from the resistant parent, Weimai 8. Meanwhile, the QTLs located on chromosomes 1B and 2AS were found close to Yr18 and Yr9, respectively. Furthermore, the results indicated that QYr.wmy-1B is possibly Yr9 and QYr.wmy-2AS is similar to Yr18 or might be a new QTL, whilst QYr.wmy-2BS and QYr.wmy-7DS were found to be different from previously reported stripe-rust-resistance QTLs and are possibly new QTLs. Overall, the QTLs and their closely associated molecular markers detected in this study could be a great source of input for marker-assisted selection to adult plant stripe-rust resistance in wheat-breeding programs.

Published in Agronomy

ISSN: 2073-4395 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Agriculture
Website: http://www.mdpi.com/journal/agronomy

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