Dual production of polyhydroxyalkanoates and antibacterial/antiviral gold nanoparticles

Alexandra Paxinou; Alexandra Paxinou; Elena Marcello; Elena Marcello; Vittoria Vecchiato; Lara Erman; Edward Wright; Brendon Noble; Adele McCormick; Adele McCormick; Pooja Basnett

doi:10.3389/fnano.2023.1243056

Frontiers in Nanotechnology (Sep 2023)

Dual production of polyhydroxyalkanoates and antibacterial/antiviral gold nanoparticles

Alexandra Paxinou,
Alexandra Paxinou,
Elena Marcello,
Elena Marcello,
Vittoria Vecchiato,
Lara Erman,
Edward Wright,
Brendon Noble,
Adele McCormick,
Adele McCormick,
Pooja Basnett

Affiliations

Alexandra Paxinou: Sustainable Biotechnology Group, School of Life Sciences, College of Liberal Arts and Sciences, University of Westminster, London, United Kingdom
Alexandra Paxinou: Foundation of Research and Technology Hellas, Institute of Chemical Engineering and High Temperature Chemical Processes (FORTH/ICE-HT), Patras, Greece
Elena Marcello: Sustainable Biotechnology Group, School of Life Sciences, College of Liberal Arts and Sciences, University of Westminster, London, United Kingdom
Elena Marcello: Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Turin, Italy
Vittoria Vecchiato: Sustainable Biotechnology Group, School of Life Sciences, College of Liberal Arts and Sciences, University of Westminster, London, United Kingdom
Lara Erman: Sustainable Biotechnology Group, School of Life Sciences, College of Liberal Arts and Sciences, University of Westminster, London, United Kingdom
Edward Wright: Viral Pseudoptype Unit, School of Life Sciences, University of Sussex, Falmer, United Kingdom
Brendon Noble: Sustainable Biotechnology Group, School of Life Sciences, College of Liberal Arts and Sciences, University of Westminster, London, United Kingdom
Adele McCormick: Sustainable Biotechnology Group, School of Life Sciences, College of Liberal Arts and Sciences, University of Westminster, London, United Kingdom
Adele McCormick: Genomics and Infection Diseases Research Group, School of Life Sciences, College of Liberal Arts and Sciences, University of Westminster, London, United Kingdom
Pooja Basnett: Sustainable Biotechnology Group, School of Life Sciences, College of Liberal Arts and Sciences, University of Westminster, London, United Kingdom

DOI: https://doi.org/10.3389/fnano.2023.1243056
Journal volume & issue: Vol. 5

Abstract

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Gold nanoparticles (AuNPs) have been explored for their use in medicine. Here, we report a sustainable, and cost-effective method to produce AuNPs using a bacterial strain such as Pseudomonas mendocina CH50 which is also known to be a polyhydroxyalkanoate (PHA) producer. A cell-free bacterial supernatant, which is typically discarded after PHA extraction, was used to produce spherical AuNPs of 3.5 ± 1.5 nm in size as determined by Transmission Electron Microscopy (TEM) analysis. The AuNPs/PHA composite coating demonstrated antibacterial activity against Staphylococcus aureus 6538P, and antiviral activity, with a 75% reduction in viral infectivity against SARS-CoV-2 pseudotype virus.

Published in Frontiers in Nanotechnology

ISSN: 2673-3013 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology
Website: https://www.frontiersin.org/journals/nanotechnology#

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