Immediately after application glyphosate is mostly adsorbed in the upper 2 cm of soils, and is then transported and adsorbed after few days in deeper soil horizons with concomitant increasing content of its metabolite aminomethylphosphonic acid (AMPA). This work confirmed previous studies, where Fe-oxides seem to play a major role in the adsorption of glyphosate and AMPA in soils: the Chernozem featured lower contents of Fed and Feo, with consequently lower adsorption of glyphosate and AMPAas compared with the higher weathered Cambisol and Stagnosol.