Bioevaluation of Novel Anti-Biofilm Coatings Based on PVP/Fe3O4 Nanostructures and 2-((4-Ethylphenoxy)methyl)-N- (arylcarbamothioyl)benzamides
Carmen Limban,
Alexandru Vasile Missir,
Alexandru Mihai Grumezescu,
Alexandra Elena Oprea,
Valentina Grumezescu,
Bogdan Ștefan Vasile,
Gabriel Socol,
Roxana Trușcă,
Miron Teodor Caproiu,
Mariana Carmen Chifiriuc,
Bianca Gălățeanu,
Marieta Costache,
Laurențiu Morușciag,
Grațiela Pîrcălăbioru,
Diana Camelia Nuță
Affiliations
Carmen Limban
Department of Pharmaceutical Chemistry, "Carol Davila" University of Medicine and Pharmacy, Traian Vuia No. 6, 020956 Bucharest, Romania
Alexandru Vasile Missir
Department of Pharmaceutical Chemistry, "Carol Davila" University of Medicine and Pharmacy, Traian Vuia No. 6, 020956 Bucharest, Romania
Alexandru Mihai Grumezescu
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1–7,011061 Bucharest, Romania
Alexandra Elena Oprea
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1–7,011061 Bucharest, Romania
Valentina Grumezescu
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1–7,011061 Bucharest, Romania
Bogdan Ștefan Vasile
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1–7,011061 Bucharest, Romania
Gabriel Socol
National Institute for Lasers, Plasma & Radiation Physics, Lasers Department, P.O. Box MG-36, Bucharest-Magurele, Romania
Roxana Trușcă
Metav-CD S.A., 31Rosetti Str., 020015 Bucharest, Romania
Miron Teodor Caproiu
The Organic Chemistry Center of Romanian Academy "Costin C.D. Nenitescu" Bucharest, Splaiul Independentei, 202B, 77208 Bucharest, Romania
Mariana Carmen Chifiriuc
Department of Microbiology, Faculty of Biology, University of Bucharest, Research Institute ofUniversity of Bucharest, Aleea Portocalelor No. 1–3, 060101 Bucharest, Romania
Bianca Gălățeanu
Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independenței, 050095 Bucharest, Romania
Marieta Costache
Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independenței, 050095 Bucharest, Romania
Laurențiu Morușciag
Department of Pharmaceutical Chemistry, "Carol Davila" University of Medicine and Pharmacy, Traian Vuia No. 6, 020956 Bucharest, Romania
Grațiela Pîrcălăbioru
Conway Institute, University College Dublin, Dublin 4, Dublin, Ireland
Diana Camelia Nuță
Department of Pharmaceutical Chemistry, "Carol Davila" University of Medicine and Pharmacy, Traian Vuia No. 6, 020956 Bucharest, Romania
Novel derivatives were prepared by reaction of aromatic amines with 2-(4-ethylphenoxymethyl)benzoyl isothiocyanate, affording the N-[2-(4-ethylphenoxymethyl) benzoyl]-Nꞌ-(substituted phenyl)thiourea. Structural elucidation of these compounds was performed by IR, NMR spectroscopy and elemental analysis. The new compounds were used in combination with Fe3O4 and polyvinylpyrrolidone (PVP) for the coating of medical surfaces. In our experiments, catheter pieces were coated by Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique. The microbial adherence ability was investigated in 6 multi-well plates by using culture based methods. The obtained surfaces were also assessed for their cytotoxicity with respect to osteoblast cells, by using fluorescence microscopy and MTT assay. The prepared surfaces by advanced laser processing inhibited the adherence and biofilm development ability of Staphylococcus aureus and Pseudomonas aeruginosa tested strains while cytotoxic effects on the 3T3-E1 preosteoblasts embedded in layer shaped alginate hydrogels were not observed. These results suggest that the obtained medical surfaces, based on the novel thiourea derivatives and magnetic nanoparticles with a polymeric shell could represent a promising alternative for the development of new and effective anti-infective strategies.
