eLife (Jan 2018)
Detection of human disease conditions by single-cell morpho-rheological phenotyping of blood
- Nicole Toepfner,
- Christoph Herold,
- Oliver Otto,
- Philipp Rosendahl,
- Angela Jacobi,
- Martin Kräter,
- Julia Stächele,
- Leonhard Menschner,
- Maik Herbig,
- Laura Ciuffreda,
- Lisa Ranford-Cartwright,
- Michal Grzybek,
- Ünal Coskun,
- Elisabeth Reithuber,
- Geneviève Garriss,
- Peter Mellroth,
- Birgitta Henriques-Normark,
- Nicola Tregay,
- Meinolf Suttorp,
- Martin Bornhäuser,
- Edwin R Chilvers,
- Reinhard Berner,
- Jochen Guck
Affiliations
- Nicole Toepfner
- Center of Molecular and Cellular Bioengineering, Biotechnology Center, Technische Universität Dresden, Dresden, Germany; Department of Medicine, University of Cambridge, Cambridge, United Kingdom; Department of Pediatrics, University Clinic Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany
- Christoph Herold
- Center of Molecular and Cellular Bioengineering, Biotechnology Center, Technische Universität Dresden, Dresden, Germany; Zellmechanik Dresden GmbH, Dresden, Germany
- Oliver Otto
- Center of Molecular and Cellular Bioengineering, Biotechnology Center, Technische Universität Dresden, Dresden, Germany; Zellmechanik Dresden GmbH, Dresden, Germany; ZIK HIKE, Universität Greifswald, Greifswald, Germany
- Philipp Rosendahl
- ORCiD
- Center of Molecular and Cellular Bioengineering, Biotechnology Center, Technische Universität Dresden, Dresden, Germany; Zellmechanik Dresden GmbH, Dresden, Germany
- Angela Jacobi
- Center of Molecular and Cellular Bioengineering, Biotechnology Center, Technische Universität Dresden, Dresden, Germany
- Martin Kräter
- ORCiD
- Department of Hematology and Oncology, University Clinic Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany
- Julia Stächele
- Department of Pediatrics, University Clinic Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany
- Leonhard Menschner
- Department of Pediatrics, University Clinic Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany
- Maik Herbig
- Center of Molecular and Cellular Bioengineering, Biotechnology Center, Technische Universität Dresden, Dresden, Germany
- Laura Ciuffreda
- Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, United Kingdom
- Lisa Ranford-Cartwright
- Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, United Kingdom
- Michal Grzybek
- Paul Langerhans Institute Dresden of the Helmholtz Centre Munich, University Hospital and Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany; German Center for Diabetes Research, Neuherberg, Germany
- Ünal Coskun
- ORCiD
- Paul Langerhans Institute Dresden of the Helmholtz Centre Munich, University Hospital and Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany; German Center for Diabetes Research, Neuherberg, Germany
- Elisabeth Reithuber
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden; Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden
- Geneviève Garriss
- ORCiD
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden; Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden
- Peter Mellroth
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden; Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden
- Birgitta Henriques-Normark
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden; Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden
- Nicola Tregay
- Department of Medicine, University of Cambridge, Cambridge, United Kingdom
- Meinolf Suttorp
- Department of Pediatrics, University Clinic Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany
- Martin Bornhäuser
- Department of Hematology and Oncology, University Clinic Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany
- Edwin R Chilvers
- ORCiD
- Department of Medicine, University of Cambridge, Cambridge, United Kingdom
- Reinhard Berner
- Department of Pediatrics, University Clinic Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany
- Jochen Guck
- ORCiD
- Center of Molecular and Cellular Bioengineering, Biotechnology Center, Technische Universität Dresden, Dresden, Germany
- DOI
- https://doi.org/10.7554/eLife.29213
- Journal volume & issue
-
Vol. 7
Abstract
Blood is arguably the most important bodily fluid and its analysis provides crucial health status information. A first routine measure to narrow down diagnosis in clinical practice is the differential blood count, determining the frequency of all major blood cells. What is lacking to advance initial blood diagnostics is an unbiased and quick functional assessment of blood that can narrow down the diagnosis and generate specific hypotheses. To address this need, we introduce the continuous, cell-by-cell morpho-rheological (MORE) analysis of diluted whole blood, without labeling, enrichment or separation, at rates of 1000 cells/sec. In a drop of blood we can identify all major blood cells and characterize their pathological changes in several disease conditions in vitro and in patient samples. This approach takes previous results of mechanical studies on specifically isolated blood cells to the level of application directly in blood and adds a functional dimension to conventional blood analysis.
Keywords
- real-time deformability cytometry
- cell mechanics
- spherocytosis
- malaria
- neutrophil activation
- leukemia
