Detoxification of Sunset yellow and Brilliant blue dyes using Soybean peroxidases

Abstract

This present study aims to evaluate the peroxidase activity in soybean extract which was obtained through the seeds of the soybean. This enzymatic extract (Soybean Peroxidase-SBP) was then compared with commercially available pure enzyme i.e., horseradish peroxidase (HRP). The effect of the enzyme concentration, substrate concentration, pH temperature were studied to interpret the factors affectingthe rate of the reaction. Therefore, after the studies were carried out, it was detected that the increase in the concentration of the substrate as well as enzyme resulted in the gradual increase of the activity. The rate of the reaction thus became constant when all the enzymes got saturated after a certain range. The optimum pH and temperature of 7 and 40 ​°C was maintained for the decolorization of the synthetic dyes viz., Sunset yellow and Coomassie Brilliant blue dye R-250. The removal of Sunset yellow dye and CBB was around 45 % and 11 %, respectively and results were compared with the degradation with HRP in similar conditions. The effective degradation of Sunset yellow over CBB may be because of the difference in the molecular weight. Furthermore, optimization of reaction mixture and reaction conditions is in progress. The results obtained in this work suggest that peroxidases can be used in a supplementary step to the conventional processes for specific dye removal.

Keywords

• Soybean peroxidase (SBP)
• Decolorization
• Sunset yellow
• Coomassie brilliant blue R-250 (CBB)
• Horseradish peroxidase (HRP)