The effect of fish oil and ozonated fish oil on the oxidative status of animals during simulation of physical load
Abstract
The objective was to study the effectiveness of fish oil (FO) and ozonated fish oil (OFO) on the intensity of lipid peroxidation (LPO) processes and the antioxidant system of the blood during physical activity «to failure».Methods and materials. The animals were divided into 4 groups of 12 rats. Saline was administered orally to control animals (group 1). Rats (group 2) were fed fish oil (dose 35 mg/kg), rats of group 3 – ozonated fish oil (dose 35 mg / kg, ozonide number 3000), group 4 – ozonated fish oil (dose 35 mg/kg, ozonide number 1500). Physical activity was modelated the method of forced swimming of rat «to failure» with a load of 10 % of body weight. The state of the LPO system was assessed by the concentration of malondialdehyde (MDA) in erythrocytes and the level of diene conjugates (DC), triene conjugates (TC) and Schiff bases (SH) in the blood plasma. The state of the antioxidant system was determined by the activity of catalase in the blood plasma.Results. Physical activity had an increase in DC, TC, OR in the blood plasma, an increase in the MDA content in erythrocytes, which was accompanied by a gradual increase in catalase activity in the blood plasma. The administration of FO against the background of physical activity determined less pronounced lipid peroxidation, while the introduction of OFO with an ozonide number of 3000 determined the most pronounced lipid peroxidation compared to the control. The lowest oxidative effect of physical compounds was recorded with the introduction of OFO with an ozonide number of 1500.Conclusions. Oral administration of OFO with an ozonide number of 1500 during physical exercise of significant intensity inhibited the development of oxidative stress against the background of high antioxidant activity of the blood to a greater extent than the use of FO.
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