Copaiba Oil-Based Emulsion as a Natural Chemotherapeutic Agent for the Treatment of Bovine Mastitis: In Vivo Studies
Katieli da Silva Souza Campanholi,
Ranulfo Combuca da Silva Junior,
Flávia Amanda Pedroso de Morais,
Renato Sonchini Gonçalves,
Bruna Moura Rodrigues,
Magali Soares dos Santos Pozza,
Lidiane Vizioli de Castro-Hoshino,
Silvio Mayke Leite,
Otávio Augusto Capeloto,
Mauro Luciano Baesso,
Paulo Cesar Pozza,
Wilker Caetano
Affiliations
Katieli da Silva Souza Campanholi
Chemistry Department, State University of Maringá, Maringá 87020-900, Brazil
Ranulfo Combuca da Silva Junior
Chemistry Department, State University of Maringá, Maringá 87020-900, Brazil
Flávia Amanda Pedroso de Morais
Centre de Recherches sur les Macromolecules Végétales (CERMAV)-CNRS, Université Grenoble Alpes, 601, rue de la Chimie, BP 53, CEDEX 9, 38041 Grenoble, France
Renato Sonchini Gonçalves
Laboratory of Chemistry of Natural Products, Department of Chemistry, Center for Exact Sciences and Technology, Federal University of Maranhão, São Luís 65080-805, Brazil
Bruna Moura Rodrigues
Animal Science Department, State University of Maringá, Maringá 87020-900, Brazil
Magali Soares dos Santos Pozza
Animal Science Department, State University of Maringá, Maringá 87020-900, Brazil
Lidiane Vizioli de Castro-Hoshino
Physics Department, State University of Maringá, Maringá 87020-900, Brazil
Silvio Mayke Leite
Animal Science Department, State University of Maringá, Maringá 87020-900, Brazil
Otávio Augusto Capeloto
Institute of Health and Biotechnology of Coari, Federal University of Amazonas, Coari 69460-000, Brazil
Mauro Luciano Baesso
Physics Department, State University of Maringá, Maringá 87020-900, Brazil
Paulo Cesar Pozza
Animal Science Department, State University of Maringá, Maringá 87020-900, Brazil
Wilker Caetano
Chemistry Department, State University of Maringá, Maringá 87020-900, Brazil
Copaiba oil-resin (COR) extracted from Copaifera reticulata Ducke has been used as a natural chemotherapeutic agent for a wide range of therapeutic applications. This study presents an emulgel design with a high concentration of COR, designed to prevent and treat mastitis. The COR was stabilized in a gel matrix constituted by carbopol C934P and Pluronic® F127 (ECO formulation) ratios. The permeation study of ECO was accessed by Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS). The results reveal a high capacity of ECO to permeate deep skin layers. Dairy cows with a history of mastitis were used as in vivo models and exposed to ECO treatment. Monitoring of the teat’s inflammatory response showed that ECO effectively prevents mastitis. Furthermore, the ECO formulation was able to form a thin film gel on the application side, preventing fly proliferation and significantly reducing the pathogen load. This study reveals a drug that can used as an alternative application for mastitis in human or veterinary clinics.
