Coluna/Columna (Dec 2013)

Two intrathecal transplants of bone marrow mononuclear cells produce motor improvement in an acute and severe model of spinal cord injury

  • Elisa Lettnin Kaminski,
  • Asdrubal Falavigna,
  • Gianina Terribele Venturin,
  • Daniel Marinowic,
  • Pamela Brambilla Bagatini,
  • Léder Leal Xavier,
  • Jaderson Costa DaCosta

DOI
https://doi.org/10.1590/S1808-18512013000400001
Journal volume & issue
Vol. 12, no. 4
pp. 274 – 277

Abstract

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OBJECTIVE: We studied transplants of bone marrow mononuclear cells (BMMC) by lumbar puncture (LP) in a severe model of spinal cord injury (SCI) using clip compression. METHODS: BMMCs or saline solution were transplanted by LP 48 hours and 9 days post injury. Motor function was evaluated by BBB scale, histological analysis by Nissl technique and the verification of cell migration by PCR analysis. RESULTS: The BBB had significantly improved in rats treated with BMMCs by LP compared with controls (p<0.001). The histological analysis did not showed difference in the lesional area between the groups. The PCR analysis was able to found BMMCs in the injury site. CONCLUSIONS: two BMMC transplants by LP improved motor function in a severe model of SCI and BMMC was found in the injury site.

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