Química Nova (Aug 2005)

Teste de TBA aplicado a carnes e derivados: métodos tradicionais, modificados e alternativos TBA test applied to meats and their products: traditional, modified and alternative methods

  • Cibele Cristina Osawa,
  • Pedro Eduardo de Felício,
  • Lireny Ap. Guaraldo Gonçalves

DOI
https://doi.org/10.1590/S0100-40422005000400019
Journal volume & issue
Vol. 28, no. 4
pp. 655 – 663

Abstract

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The TBA test is essential to quality control of fat-containing food, being the test most applied to evaluate lipid peroxidation in fishery, meat and poultry products. It estimates malonaldehyde, a secondary oxidation product, by reacting with 2-thiobarbituric acid, forming a coloured complex, measured spectrophotometrically atlambda = 532 nm. Results are expressed as mg malonaldehyde per kg sample or frequently as "TBA value". There are four ways of quantifying it: by lipid extraction, direct heating, distillation or heat-acid extraction. This review intends to point out traditional, modified and alternative TBA test methods, besides enumerating advantages and drawbacks of each one.

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