Structure and function of the metagenomic plastic-degrading polyester hydrolase PHL7 bound to its product

P. Konstantin Richter; Paula Blázquez-Sánchez; Ziyue Zhao; Felipe Engelberger; Christian Wiebeler; Georg Künze; Ronny Frank; Dana Krinke; Emanuele Frezzotti; Yuliia Lihanova; Patricia Falkenstein; Jörg Matysik; Wolfgang Zimmermann; Norbert Sträter; Christian Sonnendecker

doi:10.1038/s41467-023-37415-x

Nature Communications (Apr 2023)

Structure and function of the metagenomic plastic-degrading polyester hydrolase PHL7 bound to its product

P. Konstantin Richter,
Paula Blázquez-Sánchez,
Ziyue Zhao,
Felipe Engelberger,
Christian Wiebeler,
Georg Künze,
Ronny Frank,
Dana Krinke,
Emanuele Frezzotti,
Yuliia Lihanova,
Patricia Falkenstein,
Jörg Matysik,
Wolfgang Zimmermann,
Norbert Sträter,
Christian Sonnendecker

Affiliations

P. Konstantin Richter: Institute of Bioanalytical Chemistry, Centre for Biotechnology and Biomedicine, Leipzig University
Paula Blázquez-Sánchez: Institute of Analytical Chemistry, Leipzig University
Ziyue Zhao: Institute of Analytical Chemistry, Leipzig University
Felipe Engelberger: Institute for Drug Discovery, Leipzig University Medical School
Christian Wiebeler: Institute of Analytical Chemistry, Leipzig University
Georg Künze: Institute for Drug Discovery, Leipzig University Medical School
Ronny Frank: Centre for Biotechnology and Biomedicine, Molecular Biological-Biochemical Processing Technology, Leipzig University
Dana Krinke: Centre for Biotechnology and Biomedicine, Molecular Biological-Biochemical Processing Technology, Leipzig University
Emanuele Frezzotti: Department of Chemical Life and Environmental Sciences, University of Parma
Yuliia Lihanova: Institute of Analytical Chemistry, Leipzig University
Patricia Falkenstein: Institute of Analytical Chemistry, Leipzig University
Jörg Matysik: Institute of Analytical Chemistry, Leipzig University
Wolfgang Zimmermann: Institute of Analytical Chemistry, Leipzig University
Norbert Sträter: Institute of Bioanalytical Chemistry, Centre for Biotechnology and Biomedicine, Leipzig University
Christian Sonnendecker: Institute of Analytical Chemistry, Leipzig University

DOI: https://doi.org/10.1038/s41467-023-37415-x
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 11

Abstract

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The authors describe the cocrystal structure of the highly efficient polyethylene terephthalate-degrading hydrolase PHL7 with its product terephthalic acid and elucidate the role of residues in subsites I and II for its thermal stability and of residue L210 for its high activity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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