Immunomodulatory and Anticancer Effects of <i>Fridericia chica</i> Extract-Loaded Nanocapsules in Myeloid Leukemia
Alice de Freitas Gomes,
Adriane Dâmares de Souza Jorge Batalha,
Carlos Eduardo de Castro Alves,
Renata Galvão de Azevedo,
Jesus Rafael Rodriguez Amado,
Tatiane Pereira de Souza,
Hector Henrique Ferreira Koolen,
Felipe Moura Araújo da Silva,
Francisco Celio Maia Chaves,
Serafim Florentino Neto,
Antônio Luiz Boechat,
Gemilson Soares Pontes
Affiliations
Alice de Freitas Gomes
Post-Graduate Program in Hematology, The State University of Amazon (UEA), Foundation of Hematology and Hemotherapy of Amazonas, Manaus 69050-010, AM, Brazil
Adriane Dâmares de Souza Jorge Batalha
Laboratory of Innovative Therapies, Federal University of Amazonas (UFAM)), Manaus 69077-000, AM, Brazil
Carlos Eduardo de Castro Alves
Laboratory of Virology and Immunology, National Institute of Amazonian Research (INPA), Manaus 69067-375, AM, Brazil
Renata Galvão de Azevedo
Laboratory of Virology and Immunology, National Institute of Amazonian Research (INPA), Manaus 69067-375, AM, Brazil
Jesus Rafael Rodriguez Amado
Post-Graduate Program in Health Sciences, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados 79825-070, MS, Brazil
Tatiane Pereira de Souza
Post-Graduate Program in Pharmaceutical Innovation, Federal University of Amazonas (UFAM)), Manaus 69077-000, AM, Brazil
Hector Henrique Ferreira Koolen
Research Group in Metabolomics and Mass Spectrometry, Amazonas State University (UEA), Manaus 690065-130, AM, Brazil
Felipe Moura Araújo da Silva
Multidisciplinary Support Center, Federal University of Amazonas (UFAM)), Manaus 69080-900, AM, Brazil
Francisco Celio Maia Chaves
Embrapa Western Amazonia, Manaus 69010-970, AM, Brazil
Serafim Florentino Neto
Post-Graduate Program in Health Sciences, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados 79825-070, MS, Brazil
Antônio Luiz Boechat
Post-Graduate Program in Basic and Applied Immunology, Institute of Biological Science, Federal University of Amazonas (UFAM), Manaus 69077-000, AM, Brazil
Gemilson Soares Pontes
Post-Graduate Program in Hematology, The State University of Amazon (UEA), Foundation of Hematology and Hemotherapy of Amazonas, Manaus 69050-010, AM, Brazil
Nanocapsules provide selective delivery and increase the bioavailability of bioactive compounds. In this study, we examined the anticancer and immunomodulatory potential of Fridericia chica (crajiru) extract encapsulated in nanocapsules targeting myeloid leukemias. Nanocapsules containing crajiru (nanocapsules-CRJ) were prepared via interfacial polymer deposition and solvent displacement. Size and polydispersity were measured by dynamic light scattering. Biological assays were performed on leukemia cell lines HL60 and K562 and on non-cancerous Vero cells and human PBMC. The anticancer activity was evaluated using cytotoxicity and clonogenic assays, while the immunomodulatory activity was evaluated by measuring the levels of pro- and anti-inflammatory cytokines in PBMC supernatants treated with concentrations of nanocapsules-CRJ. Nanocapsules-CRJ exhibited significant cytotoxic activity against HL60 and K562 cells at concentrations ranging from 0.75 to 50 μg/mL, with the greatest reductions in cell viability observed at 50 μg/mL (p p p p = 0.0002), IL-10 (p = 0.0005), IL-12 (p = 0.001), and TNF-α (p = 0.005), indicating their immunomodulatory potential. These findings suggest that nanocapsules-CRJ hold promise as a potential therapeutic agent with both cytotoxic and immunomodulatory properties.
