Bioinspired core-shell nanofiber drug-delivery system modulates osteogenic and osteoclast activity for bone tissue regeneration

Shabnam Anjum; Yulin Wang; Yuan Xin; Xiao Li; Ting Li; Hengtong Zhang; Liang Quan; Ya Li; Dilip Kumar Arya; P.S. Rajinikanth; Qiang Ao

Materials Today Bio (Jun 2024)

Bioinspired core-shell nanofiber drug-delivery system modulates osteogenic and osteoclast activity for bone tissue regeneration

Shabnam Anjum,
Yulin Wang,
Yuan Xin,
Xiao Li,
Ting Li,
Hengtong Zhang,
Liang Quan,
Ya Li,
Dilip Kumar Arya,
P.S. Rajinikanth,
Qiang Ao

Affiliations

Shabnam Anjum: Department of Tissue Engineering, School of Intelligent Medicine, China Medical University, Shenyang, Liaoning, 110122, China; NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative Biomaterial & Institute of Regulatory Science for Medical Device & National Engineering Research Centre for Biomaterials, Sichuan University, Chengdu, Sichuan, 610064, China
Yulin Wang: NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative Biomaterial & Institute of Regulatory Science for Medical Device & National Engineering Research Centre for Biomaterials, Sichuan University, Chengdu, Sichuan, 610064, China
Yuan Xin: NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative Biomaterial & Institute of Regulatory Science for Medical Device & National Engineering Research Centre for Biomaterials, Sichuan University, Chengdu, Sichuan, 610064, China
Xiao Li: Department of Tissue Engineering, School of Intelligent Medicine, China Medical University, Shenyang, Liaoning, 110122, China
Ting Li: Department of Laboratory Medicine, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China
Hengtong Zhang: NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative Biomaterial & Institute of Regulatory Science for Medical Device & National Engineering Research Centre for Biomaterials, Sichuan University, Chengdu, Sichuan, 610064, China
Liang Quan: NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative Biomaterial & Institute of Regulatory Science for Medical Device & National Engineering Research Centre for Biomaterials, Sichuan University, Chengdu, Sichuan, 610064, China
Ya Li: NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative Biomaterial & Institute of Regulatory Science for Medical Device & National Engineering Research Centre for Biomaterials, Sichuan University, Chengdu, Sichuan, 610064, China
Dilip Kumar Arya: Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, Vidya Vihar, Raebareli Road, Lucknow, India
P.S. Rajinikanth: Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, Vidya Vihar, Raebareli Road, Lucknow, India
Qiang Ao: Department of Tissue Engineering, School of Intelligent Medicine, China Medical University, Shenyang, Liaoning, 110122, China; NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative Biomaterial & Institute of Regulatory Science for Medical Device & National Engineering Research Centre for Biomaterials, Sichuan University, Chengdu, Sichuan, 610064, China; Corresponding author. Department of Tissue Engineering, School of Intelligent Medicine, China Medical University, Shenyang, Liaoning, 110122, China.

Journal volume & issue: Vol. 26
p. 101088

Abstract

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Osteogenic-osteoclast coupling processes play a crucial role in bone regeneration. Recently, strategies that focus on multi-functionalized implant surfaces to enhance the healing of bone defects through the synergistic regulation of osteogenesis and osteoclastogenesis is still a challenging task in the field of bone tissue engineering. The aim of this study was to create a dual-drug release-based core-shell nanofibers with the intent of achieving a time-controlled release to facilitate bone regeneration. We fabricated core-shell P/PCL nanofibers using coaxial electrospinning, where alendronate (ALN) was incorporated into the core layer and hydroxyapatite (HA) into shell. The surface of the nanofiber construct was further modified with mussel-derived polydopamine (PDA) to induce hydrophilicity and enhance cell interactions. Surface characterizations confirmed the successful synthesis of PDA@PHA/PCL-ALN nanofibers endowed with excellent mechanical strength (20.02 ± 0.13 MPa) and hydrophilicity (22.56°), as well as the sustained sequential release of ALN and Ca ions. In vitro experiments demonstrated that PDA-functionalized core-shell PHA/PCL-ALN scaffolds possessed excellent cytocompatibility, enhanced cell adhesion and proliferation, alkaline phosphatase activity and osteogenesis-related genes. In addition to osteogenesis, the engineered scaffolds also significantly reduced osteoclastogenesis, such as tartrate-resistant acid phosphatase activity and osteoclastogenesis-related gene expression. After 12-week of implantation, it was observed that PDA@PHA/PCL-ALN nanofiber scaffolds, in a rat cranial defect model, significantly promoted bone repair and regeneration. Microcomputed tomography, histological examination, and immunohistochemical analysis collectively demonstrated that the PDA-functionalized core-shell PHA/PCL-ALN scaffolds exhibited exceptional osteogenesis-inducing and osteoclastogenesis-inhibiting effects. Finally, it may be concluded from our results that the bio-inspired surface-functionalized multifunctional, biomimetic and controlled release core-shell nanofiber provides a promising strategy to facilitate bone healing.

Published in Materials Today Bio

ISSN: 2590-0064 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General); Science: Biology (General)
Website: https://www.journals.elsevier.com/materials-today-bio

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