Endovascular biopsy: Strategy for analyzing gene expression profiles of individual endothelial cells obtained from human vessels

Zhengda Sun; Devon A. Lawson; Elizabeth Sinclair; Chih-Yang Wang; Ming-Derg Lai; Steven W. Hetts; Randall T. Higashida; Christopher F. Dowd; Van V. Halbach; Zena Werb; Hua Su; Daniel L. Cooke

doi:10.1016/j.btre.2015.07.001

Biotechnology Reports (Sep 2015)

Endovascular biopsy: Strategy for analyzing gene expression profiles of individual endothelial cells obtained from human vessels

Zhengda Sun,
Devon A. Lawson,
Elizabeth Sinclair,
Chih-Yang Wang,
Ming-Derg Lai,
Steven W. Hetts,
Randall T. Higashida,
Christopher F. Dowd,
Van V. Halbach,
Zena Werb,
Hua Su,
Daniel L. Cooke

Affiliations

Zhengda Sun: Division of Neurointerventional Radiology, Department of Radiology and Biomedical Imaging, University of California, San Francisco, CA, USA
Devon A. Lawson: Department of Anatomy, University of California, San Francisco, CA, USA
Elizabeth Sinclair: Division of Experimental Medicine, Department of Medicine, University of California, San Francisco, CA, USA
Chih-Yang Wang: Department of Anatomy, University of California, San Francisco, CA, USA
Ming-Derg Lai: Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan
Steven W. Hetts: Division of Neurointerventional Radiology, Department of Radiology and Biomedical Imaging, University of California, San Francisco, CA, USA
Randall T. Higashida: Division of Neurointerventional Radiology, Department of Radiology and Biomedical Imaging, University of California, San Francisco, CA, USA
Christopher F. Dowd: Division of Neurointerventional Radiology, Department of Radiology and Biomedical Imaging, University of California, San Francisco, CA, USA
Van V. Halbach: Division of Neurointerventional Radiology, Department of Radiology and Biomedical Imaging, University of California, San Francisco, CA, USA
Zena Werb: Department of Anatomy, University of California, San Francisco, CA, USA
Hua Su: Center for Cerebrovascular Research, Department of Anesthesia and Perioperative Care, University of California, San Francisco, CA, USA
Daniel L. Cooke: Division of Neurointerventional Radiology, Department of Radiology and Biomedical Imaging, University of California, San Francisco, CA, USA

DOI: https://doi.org/10.1016/j.btre.2015.07.001
Journal volume & issue: Vol. 7, no. C
pp. 157 – 165

Abstract

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Purpose: To develop a strategy of achieving targeted collection of endothelial cells (ECs) by endovascular methods and analyzing the gene expression profiles of collected single ECs. Methods and results: 134 ECs and 37 leukocytes were collected from four patients' intra-iliac artery endovascular guide wires by fluorescence activated cell sorting (FACS) and analyzed by single-cell quantitative RT-PCR for expression profile of 48 genes. Compared to CD45+ leukocytes, the ECs expressed higher levels (p < 0.05) of EC surface markers used on FACS and other EC related genes. The gene expression profile showed that these isolated ECs fell into two clusters, A and B, that differentially expressed 19 genes related to angiogenesis, inflammation and extracellular matrix remodeling, with cluster B ECs have demonstrating similarities to senescent or aging ECs. Conclusion: Combination of endovascular device sampling, FACS and single-cell quantitative RT-PCR is a feasible method for analyzing EC gene expression profile in vascular lesions.

Published in Biotechnology Reports

ISSN: 2215-017X (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Chemical technology: Biotechnology
Website: https://www.journals.elsevier.com/biotechnology-reports/

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