Stem Cell Research (Mar 2016)

Generation of a heterozygous knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-39

  • Deeti K. Shetty,
  • Maneesha S. Inamdar

DOI
https://doi.org/10.1016/j.scr.2015.12.037
Journal volume & issue
Vol. 16, no. 2
pp. 308 – 310

Abstract

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Ovarian carcinoma immuno-reactive antigen domain containing 1 (OCIAD1) single copy was knocked out generating an OCIAD1 heterozygous knockout human embryonic stem line named BJNhem20-OCIAD1-CRISPR-39. The line was generated using CRISPR-Cas9D10A double nickase knockout strategy (Mali et al., 2013).