Antioxidant Activities of Steviol Glycosides from Moroccan Cultivated Stevia Rebaudiana Bertoni Leaves: An In Vitro Study

Abstract

This study explores the antioxidant potential of three steviol glycosides isolated from Stevia Rebaudiana Bertoni leaves acclimatized in Morocco. Pure compounds were isolated and characterized by column chromatography, and their antioxidant activities were assessed using Ferric Reducing Antioxidant Power (FRAP) and Total Antioxidant Capacity (TAC) tests. The process of soxhlet extraction followed by column purification revealed three steviol glycosides with the following yields: steviolbioside (0.26%), rebaudioside-A (0.63%), and stevioside which exhibited the highest abundance (1.47%). Antioxidant activity tests showed that the compounds exhibited remarkable antioxidant properties, particularly in TAC test of 69.54, 72.32, and 51.6 mg AAE/1gDM for stevioside, steviolbioside, and rebaudioside-A respectively.