Bioactive Materials (Apr 2023)
A shear-thinning, ROS-scavenging hydrogel combined with dental pulp stem cells promotes spinal cord repair by inhibiting ferroptosis
- Yibo Ying,
- Zhiyang Huang,
- Yurong Tu,
- Qiuji Wu,
- Zhaoyu Li,
- Yifan Zhang,
- Huilei Yu,
- Annian Zeng,
- Hanzhi Huang,
- Jiahui Ye,
- Weiyang Ying,
- Min Chen,
- Zhiyi Feng,
- Ziyue Xiang,
- Qingsong Ye,
- Sipin Zhu,
- Zhouguang Wang
Affiliations
- Yibo Ying
- School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China
- Zhiyang Huang
- School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China
- Yurong Tu
- School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China
- Qiuji Wu
- School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China
- Zhaoyu Li
- Department of Overseas Education College, Jimei University, Xiamen, Fujian, China
- Yifan Zhang
- School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China
- Huilei Yu
- School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China
- Annian Zeng
- School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China
- Hanzhi Huang
- School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China
- Jiahui Ye
- School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China
- Weiyang Ying
- School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China; Department of Pain Medicine, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang, China
- Min Chen
- School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China
- Zhiyi Feng
- School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China
- Ziyue Xiang
- School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China
- Qingsong Ye
- Center of Regenerative Medicine, Renmin Hospital of Wuhan University, Wuhan, 460030, Hubei, China; Massachusetts General Hospital, Harvard University, Boston, 02114, USA; Corresponding author. Center of Regenerative Medicine, Renmin Hospital of Wuhan University, Wuhan, 460030, Hubei, China.
- Sipin Zhu
- School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China; Corresponding author. Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
- Zhouguang Wang
- School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China; Corresponding author. School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
- Journal volume & issue
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Vol. 22
pp. 274 – 290
Abstract
Spinal cord injury (SCI) is a serious clinical disease. Due to the deformability and fragility of the spinal cord, overly rigid hydrogels cannot be used to treat SCI. Hence, we used TPA and Laponite to develop a hydrogel with shear-thinning ability. This hydrogel exhibits good deformation, allowing it to match the physical properties of the spinal cord; additionally, this hydrogel scavenges ROS well, allowing it to inhibit the lipid peroxidation caused by ferroptosis. According to the in vivo studies, the TPA@Laponite hydrogel could synergistically inhibit ferroptosis by improving vascular function and regulating iron metabolism. In addition, dental pulp stem cells (DPSCs) were introduced into the TPA@Laponite hydrogel to regulate the ratios of excitatory and inhibitory synapses. It was shown that this combination biomaterial effectively reduced muscle spasms and promoted recovery from SCI.
