Zanco Journal of Pure and Applied Sciences (Feb 2019)

Phenotypic and Molecular Detection of Metallo-β –Lactamase Producing Pseudomonas aeruginosa Isolates From Different Clinical Infections in Erbil

  • Bakhtyiar Sh. Azeez,
  • Kamal I. Bakr

DOI
https://doi.org/10.21271/ZJPAS.31.1.7
Journal volume & issue
Vol. 31, no. 1
pp. 46 – 56

Abstract

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Metallo-β-lactamase (MBL) producing Pseudomonas aeruginosa has been documented to be a critical nosocomial infection. Itwas continuous intrinsic and acquired resistance to a various group of antimicrobial agents and its resistance ability to develop multidrug resistance lead to a severe therapeutic problem. The study aimed to identify the molecular characterisation of clinical isolates of Metallo-β -lactamase P. aeruginosain Erbil hospitals. This study was carried out during the period from October 2017 to March 2018. A total of 300 clinical specimens were collected from patients (urine 124, wound 80, burns 40, bronchial wash 30, and sputum 26) aged 15-65 years attending Rizgary, West emergency, Erbil teaching hospitals. Out of 300 specimens, 50 isolates of P. aeruginosawere recovered and accounted for 16% of hospitalised infection isolates, the diagnosis of P. aeruginosaisolates was confirmed phenotypically and genotypically via the amplification of 16SrRNAgeneby using PCR technique. All isolates were tested toward the different class of antimicrobials by using agar diffusion method and VITEK 2 system. Levofloxacin, Norfloxacin, and Imipenem was the most effective antimicrobial, and most of the isolates were high resistance to (P, L,V, PI, R, CHL, E, B, A, N, TE, G, MEM, CEF, CTX, ATM).The lowest resistance was to IMP, LEV and NOR. Out of 50 of isolates,14 (28%) were found to produce MBL.16SrRNAwere used to confirm P. aeruginosa and blaVIM, blaIMP used to detect the MBL. All isolates were positive for 16SrRNA, while 12 (85%) and 8 (57%) were positive for blaVIM and blaIMP genes. In conclusion, the present study proved thatMetallo-β-lactamase is producing P. aeruginosaisolated had phenotypic characterisation which strongly correlated with according to genotypic characterisation. To our knowledge, this is the first attempt in Erbil city.

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