Stress-associated developmental reprogramming in moss protonemata by synthetic activation of the common symbiosis pathway
Thomas J. Kleist,
Anthony Bortolazzo,
Zachary P. Keyser,
Adele M. Perera,
Thomas B. Irving,
Muthusubramanian Venkateshwaran,
Fatiha Atanjaoui,
Ren-Jie Tang,
Junko Maeda,
Heather N. Cartwright,
Michael L. Christianson,
Peggy G. Lemaux,
Sheng Luan,
Jean-Michel Ané
Affiliations
Thomas J. Kleist
Department of Plant & Microbial Biology, University of California-Berkeley, Berkeley, CA 94720, USA; Department of Plant Biology, Carnegie Institute for Science, Stanford, CA 94305, USA; Institute for Molecular Physiology, Department of Biology, Heinrich Heine University, Düsseldorf 40225, Germany; Corresponding author
Anthony Bortolazzo
Laboratory of Genetics, University of Wisconsin-Madison, Madison, WI 53706, USA; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA
Zachary P. Keyser
Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA
Adele M. Perera
Department of Plant & Microbial Biology, University of California-Berkeley, Berkeley, CA 94720, USA
Thomas B. Irving
Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA
Muthusubramanian Venkateshwaran
School of Agriculture, University of Wisconsin-Platteville, Platteville, WI 53818, USA
Fatiha Atanjaoui
Institute for Molecular Physiology, Department of Biology, Heinrich Heine University, Düsseldorf 40225, Germany
Ren-Jie Tang
Department of Plant & Microbial Biology, University of California-Berkeley, Berkeley, CA 94720, USA
Junko Maeda
Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA
Heather N. Cartwright
Department of Plant Biology, Carnegie Institute for Science, Stanford, CA 94305, USA
Michael L. Christianson
Department of Plant & Microbial Biology, University of California-Berkeley, Berkeley, CA 94720, USA
Peggy G. Lemaux
Department of Plant & Microbial Biology, University of California-Berkeley, Berkeley, CA 94720, USA
Sheng Luan
Department of Plant & Microbial Biology, University of California-Berkeley, Berkeley, CA 94720, USA
Jean-Michel Ané
Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA; Department of Agronomy, University of Wisconsin-Madison, Madison, WI 53706, USA; Corresponding author
Summary: Symbioses between angiosperms and rhizobia or arbuscular mycorrhizal fungi are controlled through a conserved signaling pathway. Microbe-derived, chitin-based elicitors activate plant cell surface receptors and trigger nuclear calcium oscillations, which are decoded by a calcium/calmodulin-dependent protein kinase (CCaMK) and its target transcription factor interacting protein of DMI3 (IPD3). Genes encoding CCaMK and IPD3 have been lost in multiple non-mycorrhizal plant lineages yet retained among non-mycorrhizal mosses. Here, we demonstrated that the moss Physcomitrium is equipped with a bona fide CCaMK that can functionally complement a Medicago loss-of-function mutant. Conservation of regulatory phosphosites allowed us to generate predicted hyperactive forms of Physcomitrium CCaMK and IPD3. Overexpression of synthetically activated CCaMK or IPD3 in Physcomitrium led to abscisic acid (ABA) accumulation and ectopic development of brood cells, which are asexual propagules that facilitate escape from local abiotic stresses. We therefore propose a functional role for Physcomitrium CCaMK-IPD3 in stress-associated developmental reprogramming
