Folia Histochemica et Cytobiologica (Apr 2010)

The CD271 expression could be alone for establisher phenotypic marker in Bone Marrow derived mesenchymal stem cells

  • Edgardo Flores-Torales,
  • Arturo Orozco-Barocio,
  • Oscar R. Gonzalez-Ramella,
  • Antonio Carrasco-Yalan,
  • Karlen Gazarian,
  • Silvio Cuneo-Pareto

Journal volume & issue
Vol. 48, no. 4
pp. 682 – 686

Abstract

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Mesenchymal stem cells (MSCs) are of great interest for their potential use in cellular therapies. To define thepopulation more precisely, diverse surface markers have been used. We propose here to use CD271 as the sole marker forMSCs in fresh bone marrow. We compared CD271+ populations to the presence or absence of five defined markers forMSCs: CD90+, CD105+, CD45-, CD34- and CD79. The correlations between markers were evaluated and analyzed with aPearson's correlation test. We found that the average percentage of cells expressing the combination of markers CD90+,CD105+, CD45-, CD34- and CD79- was 0.54%, and that the average percentage average of CD271+ cells was 0.53%. Theresults were significant (p<0.05). The exclusive use of CD271 as a marker for MSCs from fresh samples of bone marrowappears to be highly selective. Using CD271 as the sole identification marker for MSCs could reduce costs and acceleratethe process of identifying MSCs for the field of cellular therapy.

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