Frontiers in Cell and Developmental Biology (Nov 2022)
Construction of a mouse model that can be used for tissue-specific EV screening and tracing in vivo
- Weili Li,
- Weili Li,
- Jin Wang,
- Jin Wang,
- Xiaojiao Yin,
- Huanhuan Shi,
- Huanhuan Shi,
- Benben Sun,
- Mengru Ji,
- Mengru Ji,
- Huichen Song,
- Huichen Song,
- Jiachen Liu,
- Yihao Dou,
- Chenghong Xu,
- Xiaohong Jiang,
- Xiaohong Jiang,
- Jing Li,
- Jing Li,
- Liang Li,
- Liang Li,
- Chen-Yu Zhang,
- Chen-Yu Zhang,
- Yujing Zhang,
- Yujing Zhang
Affiliations
- Weili Li
- State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, China
- Weili Li
- Chinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, China
- Jin Wang
- State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, China
- Jin Wang
- Chinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, China
- Xiaojiao Yin
- State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, China
- Huanhuan Shi
- State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, China
- Huanhuan Shi
- Chinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, China
- Benben Sun
- State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, China
- Mengru Ji
- State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, China
- Mengru Ji
- Chinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, China
- Huichen Song
- State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, China
- Huichen Song
- Chinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, China
- Jiachen Liu
- State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, China
- Yihao Dou
- State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, China
- Chenghong Xu
- State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, China
- Xiaohong Jiang
- State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, China
- Xiaohong Jiang
- Chinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, China
- Jing Li
- State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, China
- Jing Li
- Chinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, China
- Liang Li
- State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, China
- Liang Li
- Chinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, China
- Chen-Yu Zhang
- State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, China
- Chen-Yu Zhang
- Chinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, China
- Yujing Zhang
- State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, China
- Yujing Zhang
- Chinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, China
- DOI
- https://doi.org/10.3389/fcell.2022.1015841
- Journal volume & issue
-
Vol. 10
Abstract
Extracellular vesicles (EVs) play an important role in the communication between tissues and cells. However, it is difficult to screen and trace EVs secreted by specific tissues in vivo, which affects the functional study of EVs in certain tissues under pathophysiological conditions. In this study, a Cre-dependent CD63flag-EGFP co-expressed with mCherry protein system expressing mice was constructed, which can be used for the secretion, movement, and sorting of EVs from specific tissues in vivo. This mouse model is an ideal research tool for studying the secretion amount, target tissue, and functional molecule screening of EVs in specific tissues under different pathophysiological conditions. Moreover, it provides a new research method to clarify the mechanism of secreted EVs in the pathogenesis of the disease.
Keywords
