Role of Nigella Sativa Seeds on Pyrazinamide Induced Hepatotoxicity

Abstract

Objective: To determine the hepatoprotective effect of Nigella sativa seeds in Pyrazinamide induced hepatic damage in albino mice. Study Design: An experimental study. Place and Duration of Study: The study was conducted in pharmacology department of Islamic International Medical College, Riphah International University, in collaboration with National Institute of Health, Armed Force Institute of Pathology and Pakistan Institute of Medical Sciences Islamabad, Pakistan. Materials and Methods: A total of 68 male albino mice were included in the study. They were divided into 4 group's i.e, seventeen mice in each group. Group A (control group), Group B (Pyrazinamide only treated), Group C (Pyrazinamide + Low dose Nigella sativa treated) and Group D (Pyrazinamide+ High dose Nigella sativa treated). Group B, C and D received Pyrazinamide through gavage needle once daily for six weeks. Group C and D received Nigella sativa along with Pyrazinamide through gavage needle once daily for six weeks. Blood sampling (baseline, end of 3 and 6 weeks) was done to analyze serum ALT and AST levels. Data was analyzed by using SPSS version 21. Results: Pyrazinamide treatment for 6 weeks increased serum ALT and AST levels. Nigella sativa administration along with Pyrazinamide for six weeks decreased the elevated levels of ALT and AST in C and D groups. Conclusion: Nigella sativa seeds exerted hepatoprotective effect by decreasing the raised levels of ALT and AST in Pyrazinamide treated albino mice.

Keywords

• alt, ast, hepatotoxicity, nigella sativa seeds, pyrazinamide.