Changes in Number and Antibacterial Activity of Silver Nanoparticles on the Surface of Suture Materials during Cyclic Freezing
Alexander Basov,
Stepan Dzhimak,
Mikhail Sokolov,
Vadim Malyshko,
Arkadii Moiseev,
Elena Butina,
Anna Elkina,
Mikhail Baryshev
Affiliations
Alexander Basov
Department of Fundamental and Clinical Biochemistry, Kuban State Medical University, 4 Mitrofan Sedina St., 350063 Krasnodar, Russia
Stepan Dzhimak
Department of Radiophysics and Nanothechnology, Kuban State University, 149 Stavropolskaya St., 350040 Krasnodar, Russia
Mikhail Sokolov
Department of Radiophysics and Nanothechnology, Kuban State University, 149 Stavropolskaya St., 350040 Krasnodar, Russia
Vadim Malyshko
Department of Fundamental and Clinical Biochemistry, Kuban State Medical University, 4 Mitrofan Sedina St., 350063 Krasnodar, Russia
Arkadii Moiseev
Department of Organization and Support of Scientific Activities, Kuban State Agrarian University, 13 Kalinina St., 350004 Krasnodar, Russia
Elena Butina
Department of Technology of Fats, Cosmetics, Commodity Science, Processes and Devices, Kuban State Technological University, 2 Moscow St., 350072 Krasnodar, Russia
Anna Elkina
Department of Radiophysics and Nanothechnology, Kuban State University, 149 Stavropolskaya St., 350040 Krasnodar, Russia
Mikhail Baryshev
Department of Radiophysics and Nanothechnology, Kuban State University, 149 Stavropolskaya St., 350040 Krasnodar, Russia
This article presents the results of the 10-fold cyclic freezing (−37.0 °C) and thawing (0.0 °C) effect on the number and size range of silver nanoparticles (AgNPs). AgNPs were obtained by the cavitation-diffusion photochemical reduction method and their sorption on the fiber surface of various suture materials, perlon, silk, and catgut, was studied. The distribution of nanoparticles of different diameters before and after the application of the cyclic freezing/thawing processes for each type of fibers studied was determined using electron microscopy. In general, the present study demonstrates the effectiveness of using the technique of 10-fold cyclic freezing. It is applicable to increase the absolute amount of AgNPs on the surface of the suture material with a simultaneous decrease in the size dispersion. It was also found that the application of the developed technique leads to the overwhelming predominance of nanoparticles with 1 to 15 nm diameter on all the investigated fibers. In addition, it was shown that after the application of the freeze/thaw method, the antibacterial activity of silk and catgut suture materials with AgNPs was significantly higher than before their treatment by cyclic freezing.
