BioTechniques (Mar 2011)

High transfection efficiency of neural stem cells with magnetofection

  • Cédric Sapet,
  • Nicolas Laurent,
  • Antoine de Chevigny,
  • Loïc Le Gourrierec,
  • Elodie Bertosio,
  • Olivier Zelphati,
  • Christophe Béclin

DOI
https://doi.org/10.2144/000113628
Journal volume & issue
Vol. 50, no. 3
pp. 187 – 189

Abstract

Read online

Primary neural stem cells (NSCs) can be cultivated and differentiated in vitro but are difficult to transfect using conventional methods. We describe a simple and rapid magnetofection-based method suitable for the lab bench as well as for high-throughput projects. Our method yields high transfection efficiency and can be used for deciphering the genetic control of neural cell differentiation.

Keywords