Communications Biology (Oct 2022)
Live slow-frozen human tumor tissues viable for 2D, 3D, ex vivo cultures and single-cell RNAseq
- Gaetana Restivo,
- Aizhan Tastanova,
- Zsolt Balázs,
- Federica Panebianco,
- Maren Diepenbruck,
- Caner Ercan,
- Bodgan-T. Preca,
- Jürg Hafner,
- Walter P. Weber,
- Christian Kurzeder,
- Marcus Vetter,
- Simone Münst Soysal,
- Christian Beisel,
- Mohamed Bentires-Alj,
- Salvatore Piscuoglio,
- Michael Krauthammer,
- Mitchell P. Levesque
Affiliations
- Gaetana Restivo
- Department of Dermatology, University Hospital Zurich, University of Zurich
- Aizhan Tastanova
- Department of Dermatology, University Hospital Zurich, University of Zurich
- Zsolt Balázs
- Department of Quantitative Biomedicine, University Hospital Zurich
- Federica Panebianco
- Department of Biomedicine, Department of Surgery, University Hospital Basel, University of Basel
- Maren Diepenbruck
- Department of Biomedicine, Department of Surgery, University Hospital Basel, University of Basel
- Caner Ercan
- Institute of Medical Genetics and Pathology, University Hospital Basel
- Bodgan-T. Preca
- Department of Biomedicine, Department of Surgery, University Hospital Basel, University of Basel
- Jürg Hafner
- Department of Dermatology, University Hospital Zurich, University of Zurich
- Walter P. Weber
- Department of Surgery, Breast Center, University Hospital Basel
- Christian Kurzeder
- Department of Surgery, Breast Center, University Hospital Basel
- Marcus Vetter
- Department of Surgery, Breast Center, University Hospital Basel
- Simone Münst Soysal
- Department of Surgery, Breast Center, University Hospital Basel
- Christian Beisel
- Department of Biosystems Science and Engineering, ETH Zurich
- Mohamed Bentires-Alj
- Department of Biomedicine, Department of Surgery, University Hospital Basel, University of Basel
- Salvatore Piscuoglio
- Department of Biomedicine, Department of Surgery, University Hospital Basel, University of Basel
- Michael Krauthammer
- Department of Quantitative Biomedicine, University Hospital Zurich
- Mitchell P. Levesque
- Department of Dermatology, University Hospital Zurich, University of Zurich
- DOI
- https://doi.org/10.1038/s42003-022-04025-0
- Journal volume & issue
-
Vol. 5,
no. 1
pp. 1 – 12
Abstract
Fresh vs. slow-frozen tissues from various malignancies are compared for the establishment of 2D, 3D and ex vivo cultures, as well as for scRNAseq analysis, and found to be comparable and suitable for cancer research.
