Comparative Analysis of the Genomic DNA Isolation Methods on <i>Inula</i> sp. (Asteraceae)

Notulae Scientia Biologicae. 2016;8(4):451-455 DOI 10.15835/nsb849881

 

Journal Homepage

Journal Title: Notulae Scientia Biologicae

ISSN: 2067-3205 (Print); 2067-3264 (Online)

Publisher: University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca

Society/Institution: Horticulture and Forestry Society from Transylvania (SHST)

LCC Subject Category: Agriculture: Agriculture (General) | Science: Science (General)

Country of publisher: Romania

Language of fulltext: English

Full-text formats available: PDF

 

AUTHORS

Emre SEVİNDİK
Fatih COŞKUN
Zehra Tuğba MURATHAN
Mehmet Yavuz PAKSOY
Veysel UZUN

EDITORIAL INFORMATION

Double blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 12 weeks

 

Abstract | Full Text

Simple, fast, low-cost and high throughput protocols are required for DNA isolation of plant species. In this study, phenol chloroform isoamyl alcohol and commercial (Sigma) DNA isolation kit methods were applied on some Inula species that belong to Asteraceae family. Genomic DNA amounts, A260, A280, A260/A230 and purity degrees (A260/A280) that were obtained through both methods were measured through electrophoresis and spectrophotometer. Additionally, PCR amplification was realized by primer pairs specific to nrDNA ITS, cpDNA ndhF (972F-1603R) and trnL-F regions. Results showed that maximum genomic DNA in nanograms obtained by phenol chloroform isoamyl alcohol method. The study also revealed that I. macrocephala had the maximum DNA and I. heterolepis had the minimum DNA amount. A260/A280 purity degrees showed that the highest and lowest purity in gDNAs obtained through phenol-choloform isoamyl alcohol method were in I.aucheriana and I. salicina, respectively. The highest and lowest purity degrees of gDNAs obtained through commercial kit was observed in I. fragilis and I. macrocephala samples, respectively. PCR amplification results showed that while band profiles of each three regions (ITS, trnL-F and ndhF) did not yield positive results in PCR amplifications using phenol-choloform isoamyl alcohol method; PCR band profiles obtained through commercial kit yielded positive results. As a result, it is fair to say that the relation of genomic DNA with PCR was found to be more efficient although the maximum amount of genomic DNA was obtained through phenol chloroform isoamyl alcohol method.