Frontiers in Veterinary Science (Dec 2021)

Interference With ACSL1 Gene in Bovine Adipocytes: Transcriptome Profiling of mRNA and lncRNA Related to Unsaturated Fatty Acid Synthesis

  • Yanbin Bai,
  • Xupeng Li,
  • Zongchang Chen,
  • Jingsheng Li,
  • Hongshan Tian,
  • Yong Ma,
  • Sayed Haidar Abbas Raza,
  • Bingang Shi,
  • Xiangmin Han,
  • Yuzhu Luo,
  • Jiang Hu,
  • Jiqing Wang,
  • Xiu Liu,
  • Shaobin Li,
  • Zhidong Zhao

DOI
https://doi.org/10.3389/fvets.2021.788316
Journal volume & issue
Vol. 8

Abstract

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The enzyme long-chain acyl-CoA synthetase 1 (ACSL1) is essential for lipid metabolism. The ACSL1 gene controls unsaturated fatty acid (UFA) synthesis as well as the formation of lipid droplets in bovine adipocytes. Here, we used RNA-Seq to determine lncRNA and mRNA that regulate UFA synthesis in bovine adipocytes using RNA interference and non-interference with ACSL1. The corresponding target genes of differentially expressed (DE) lncRNAs and the DE mRNAs were found to be enriched in lipid and FA metabolism-related pathways, according to GO and KEGG analyses. The differentially expressed lncRNA- differentially expressed mRNA (DEL-DEM) interaction network indicated that some DELs, such as TCONS_00069661, TCONS_00040771, TCONS_ 00035606, TCONS_00048301, TCONS_001309018, and TCONS_00122946, were critical for UFA synthesis. These findings assist our understanding of the regulation of UFA synthesis by lncRNAs and mRNAs in bovine adipocytes.

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