Arthropods (Dec 2016)
Analysis of Sogatella furcifera (Horvath) soluble proteins by SDS-PAGE
Abstract
The soluble proteins from nymphs and adults of Sogatella furcifera were analyzed by SDS-PAGE. The number of protein bands increased gradually as the nymphs developing, such as six and 14 protein bands were found in 3rd-instar nymphs and 5th-instar nymphs respectively. At the same time, we found that three bands expressed in each instar, two bands began to appear from 4th-instar, and six bands were specific in 5th-instar. There were four bands that their content in 5th-instar nymphs with long-winged disc was at least 65.61% higher than in 5th-instar nymphs with short-winged disc. There were 13 protein bands observed in male adults, while female adults had 13 corresponding protein bands and a specific band expressed only in tissue. Comparing between two wing-type adults, four bands were specific to long-winged adults, while the content of other three bands in long-winged adults was at least 72.54 % higher than in short-winged adults. Finally, these specific protein bands associated with wing or sex were discussed what kind role they played in wing or sexual differentiation. The results will be helpful to further explore the mechanism of wing or sexual differentiation about planthoppers.
