Communications Biology (Nov 2021)
Picroscope: low-cost system for simultaneous longitudinal biological imaging
- Victoria T. Ly,
- Pierre V. Baudin,
- Pattawong Pansodtee,
- Erik A. Jung,
- Kateryna Voitiuk,
- Yohei M. Rosen,
- Helen Rankin Willsey,
- Gary L. Mantalas,
- Spencer T. Seiler,
- John A. Selberg,
- Sergio A. Cordero,
- Jayden M. Ross,
- Marco Rolandi,
- Alex A. Pollen,
- Tomasz J. Nowakowski,
- David Haussler,
- Mohammed A. Mostajo-Radji,
- Sofie R. Salama,
- Mircea Teodorescu
Affiliations
- Victoria T. Ly
- Department of Electrical and Computer Engineering, University of California Santa Cruz
- Pierre V. Baudin
- Department of Electrical and Computer Engineering, University of California Santa Cruz
- Pattawong Pansodtee
- Department of Electrical and Computer Engineering, University of California Santa Cruz
- Erik A. Jung
- Department of Electrical and Computer Engineering, University of California Santa Cruz
- Kateryna Voitiuk
- Department of Biomolecular Engineering, University of California Santa Cruz
- Yohei M. Rosen
- Department of Biomolecular Engineering, University of California Santa Cruz
- Helen Rankin Willsey
- Department of Psychiatry and Behavioral Sciences, Weill Institute for Neurosciences, University of California San Francisco
- Gary L. Mantalas
- Department of Molecular, Cell and Developmental Biology, University of California Santa Cruz
- Spencer T. Seiler
- Department of Biomolecular Engineering, University of California Santa Cruz
- John A. Selberg
- Department of Electrical and Computer Engineering, University of California Santa Cruz
- Sergio A. Cordero
- Department of Electrical and Computer Engineering, University of California Santa Cruz
- Jayden M. Ross
- The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco
- Marco Rolandi
- Department of Electrical and Computer Engineering, University of California Santa Cruz
- Alex A. Pollen
- The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco
- Tomasz J. Nowakowski
- The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco
- David Haussler
- Department of Biomolecular Engineering, University of California Santa Cruz
- Mohammed A. Mostajo-Radji
- The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco
- Sofie R. Salama
- Department of Biomolecular Engineering, University of California Santa Cruz
- Mircea Teodorescu
- Department of Electrical and Computer Engineering, University of California Santa Cruz
- DOI
- https://doi.org/10.1038/s42003-021-02779-7
- Journal volume & issue
-
Vol. 4,
no. 1
pp. 1 – 11
Abstract
Ly et al. report a multi-well imaging system (the Picroscope) that can perform longitudinal bright-field z-stack imaging for 24 wells simultaneously. They demonstrate its capability by imaging zebrafish embryos, planaria worms, development of Xenopus tropicalis over a 28-h period and embryonic stem cell monolayer and organoids inside an incubator.
