Effects of basal medium and hormone treatments on shoot proliferation in Rosa damascena Mill

Abstract

Damask rose is one of the most important medicinal and aromatic plants from economical point of view in Iran. Damask rose is commonly propagated by asexual methods. Conventional propagation of Rosa damascena is limited attributed to low adventitious root formation on cutting. In vitro culture has became an alternative method for propagation of this plant species. The shoot single node segments included lateral buds were taken from bushes. Single-node explants were surface sterilized with sodium hypochlorite 1.2% for 15 min and cultured on MS and QL media supplemented with various concentrations of N6- benzyl adenine (BA) and kinetin (Kin). Shoot proliferation was superior on QL than in MS medium. The best growth regulator combination were 5mgl-1 BA and 1.5mgl-1 Kin.

Keywords

• Plant growth regulators
• Proliferation
• Propagation
• Rosa damascena Mill