The bZIP transcription factor ATF1 regulates blue light and oxidative stress responses in Trichoderma guizhouense
Yifan Li,
Yanshen Li,
Huanhong Lu,
Tingting Sun,
Jia Gao,
Jian Zhang,
Qirong Shen,
Zhenzhong Yu
Affiliations
Yifan Li
Nanjing Agricultural University, Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic‐based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization Agricultural Microbial Resources Protection and Germplasm Innovation and Utilization Center of Jiangsu Province Nanjing China
Yanshen Li
Nanjing Agricultural University, Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic‐based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization Agricultural Microbial Resources Protection and Germplasm Innovation and Utilization Center of Jiangsu Province Nanjing China
Huanhong Lu
Nanjing Agricultural University, Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic‐based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization Agricultural Microbial Resources Protection and Germplasm Innovation and Utilization Center of Jiangsu Province Nanjing China
Tingting Sun
Nanjing Agricultural University, Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic‐based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization Agricultural Microbial Resources Protection and Germplasm Innovation and Utilization Center of Jiangsu Province Nanjing China
Jia Gao
Department of Microbiology Karlsruhe Institute of Technology (KIT)—South Campus, Institute for Applied Biosciences Karlsruhe Germany
Jian Zhang
Nanjing Agricultural University, Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic‐based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization Agricultural Microbial Resources Protection and Germplasm Innovation and Utilization Center of Jiangsu Province Nanjing China
Qirong Shen
Nanjing Agricultural University, Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic‐based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization Agricultural Microbial Resources Protection and Germplasm Innovation and Utilization Center of Jiangsu Province Nanjing China
Zhenzhong Yu
Nanjing Agricultural University, Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic‐based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization Agricultural Microbial Resources Protection and Germplasm Innovation and Utilization Center of Jiangsu Province Nanjing China
Abstract In several filamentous fungi, incident light and environmental stress signaling share the mitogen‐activated protein kinase (MAPK) HOG (SAK) pathway. It has been revealed that short‐term illumination with blue light triggers the activation of the HOG pathway in Trichoderma spp. In this study, we demonstrate the crucial role of the basic leucine zipper transcription factor ATF1 in blue light responses and signaling downstream of the MAPK HOG1 in Trichoderma guizhouense. The lack of ATF1 severely impaired photoconidiation and delayed vegetative growth and conidial germination. Upon blue light or H2O2 stimuli, HOG1 interacted with ATF1 in the nucleus. Genome‐wide transcriptome analyses revealed that 61.8% (509 out of 824) and 85.2% (702 out of 824) of blue light‐regulated genes depended on ATF1 and HOG1, respectively, of which 58.4% (481 out of 824) were regulated by both of them. Our results also show that blue light promoted conidial germination and HOG1 and ATF1 played opposite roles in controlling conidial germination in the dark. Additionally, the lack of ATF1 led to reduced oxidative stress resistance, probably because of the downregulation of catalase‐encoding genes. Overall, our results demonstrate that ATF1 is the downstream component of HOG1 and is responsible for blue light responses, conidial germination, vegetative growth, and oxidative stress resistance in T. guizhouense.
