Antigenotoxic Effect of Piperine in Broiler Chickens Intoxicated with Aflatoxin B1
Verônica da Silva Cardoso,
Alane Beatriz Vermelho,
Cristina Amorim Ribeiro de Lima,
Jéssica Mendes de Oliveira,
Marco Edilson Freire de Lima,
Lúcia Helena Pinto da Silva,
Glória Maria Direito,
Maria das Graças Miranda Danelli
Affiliations
Verônica da Silva Cardoso
BIOINOVAR—Biotecnologia, Unidade de Biocatálise, Bioprodutos e Bioenergia, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-901, Brazil
Alane Beatriz Vermelho
BIOINOVAR—Biotecnologia, Unidade de Biocatálise, Bioprodutos e Bioenergia, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-901, Brazil
Cristina Amorim Ribeiro de Lima
Instituto de Zootecnia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil
Jéssica Mendes de Oliveira
Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil
Marco Edilson Freire de Lima
Instituto de Ciências Exatas, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil
Lúcia Helena Pinto da Silva
Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil
Glória Maria Direito
Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil
Maria das Graças Miranda Danelli
Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil
Piperine is an abundant amide extracted from black pepper seeds which has been shown to have protective effects against cytotoxic and genotoxic carcinogenesis induced by certain chemical carcinogens and aflatoxin B1 (AFB1) in vitro. The aim of this work was to study, in vivo, the antigenotoxic potential of feed-added piperine on broiler chickens experimentally intoxicated with AFB1, using micronucleus and comet assays. The antigenotoxicity assessment of 9-day-old chicks was performed on a total of 60 chickens divided into four groups of 15 broilers each: (C) control, (P) 60 mg·piperine kg−1 feed, (A) 0.5 mg·AFB1·kg−1 body weight, (daily by oral route), and (P + A) co-treatment with piperine and AFB1. The experiment was conducted for 26 days. Chicks intoxicated with AFB1 showed significant genotoxic effects in the first 24 h post intoxication, and the effects remained in the other periods analyzed (48, 72, and 96 h and 26 days of treatment). The DNA damage in peripheral blood cells, the number of erythrocytes with micronuclei, and polychromatic-to-normochromatic erythrocyte ratio were significantly reduced or absent in the piperine/AFB1 group. No significant differences were observed between the group piperine/AFB1 and the control and piperine-alone groups. The addition 60 mg·kg−1 of piperine to the diet of the broiler chicks was safe, promoting beneficial effects in poultry health with respect to the toxic effects 0.5 mg·AFB1·kg−1 body weight.
