Molecular identification of Fasciola species based on ribosomal and mitochondrial DNA sequences in Riyadh, Saudi Arabia

Abstract

ABSTRACT Fasciola species are the causative agents of liver fluke disease (fasciolosis) which is primarily found in domestic and wild ruminants but also are causal agents of fascioliasis in humans. Few reports for the genotyping of Fasciola species in Saudi Arabia were available. This study aimed to identify the Fasciola species infecting sheep using morphology and molecular tools. For that, livers of 500 imported sheep were slaughtered at abattoirs located in Riyadh (Saudi Arabia), and parasite species were isolated from 28 liver samples and then preserved for subsequent stages in this study. These parasite species were identified as Fasciola hepatica and Fasciolagigantica via morphology and confirmed via the 28S rRNA, ITS-1, COI, and NDI sequences with a close identity for other Fasciola species within the family Fasciolidae. Phylogenetic analysis of the obtained sequences showed that Fasciola isolates from the current study were clustered in one subclade closely related to isolates from Iran, Vietnam, Australia, South Africa, Eastern Europe, India, Egypt, Sudan, Japan, Poland, Spain, Armenia, and Turkey. A combination of data from morphology and molecular analysis could be considered a useful tool for identifying Fasciola species infecting sheep in Saudi Arabia.

Keywords

• sheep
• Fasciola species
• morphology
• molecular analysis