Antibiotic Resistance of Vibrio cholera Strains Isolated in Kazakhstan

Abstract

In this research work 52 collection strains of Vibrio cholerae of different serological groups (O1, O139 and non O1) isolated in Almaty city, Almaty, Zhambyl and Kyzylorda regions of the Republic of Kazakhstan from people and from water samples taken from open reservoirs in different years (1993-2014) were used. The sensitivity of V.cholerae strains to 11 antibacterial drugs was studied. In order to assess the sensitivity to antibacterial drugs we used a disc-diffusion method according to the International Standard of the Institute of Clinical and Laboratory Standards. The studied isolates (38.4%) had phenotypic resistance markers, and the strains with mono-resistant features prevailed (23.1%); 9.6% of the studied strains had two or more resistant markers. Phenotypic resistance to kanamycin was more frequent (17.3%). Among the isolates of Vxholerae O1 serogroup, the proportion of resistant to antibiotics was 38.5%, among V.cholerae non O1 serogroup - 40%. The share of resistant strains isolated in the isolates Kyzylorda region was significant and amounted to 45.8%. Resistant strains, isolated from water in Almaty city, Almaty and Zhambyl oblasts, had one phenotype resistant to erythromycin. Modeling the incidence of multi-resistance strains of V.cholerae did not show their statistical significance. There are no differences in relation to antibiotics for serogroups.

Keywords

• cholera strains
• antibiotics
• resistance
• sensitivity
• statistics