ISAC multiplex testing – results of examination in 100 patients suffering from atopic dermatitis

Abstract

To find the molecular components with the highest underlying probability of sensitization in patients suffering from atopic dermatitis and in subgroup of patients with allergic rhinitis, asthma bronchiale and to determine whether there is some difference of sensitization in mild, moderate and severe form of AD, in patients suffering from asthma bronchiale and allergic rhinitis. The complete dermatological and allergological examination including the examination of the sensitization to molecular allergens with Multiplex ISAC testing was performed. Altogether 100 atopic dermatitis patients were examined – 48 men, 52 women, the average age 40.9 years; the sensitization to molecular components was confirmed in 93% of patients. The highest observed sensitization rate was 61.0% to grass specific molecule Phl p 1, the second most frequent sensitization was 57.0% to Betulaceae-specific molecule Bet v 1. Frequently observed sensitizations were those to PR-10 proteins, NPC2 proteins family, Uteroglobin and Lipocalin. In severe form of atopic dermatitis sensitization to molecular components of NPC2 proteins family, Uteroglobin, Lipocalin and Aspergillus were recorded with a significant higher occurrence.

Keywords

• atopic dermatitis
• molecular components
• severity of atopic dermatitis
• asthma bronchiale
• allergic rhinitis
• underlying probability of sensitization