Croatian Journal of Fisheries (Jan 2013)
POPULATION GENETIC STRUCTURE OF AN ENDANGERED KALIBAUS, Labeo calbasu (HAMILTON, 1822) REVEALED BY MICROSATELLITE DNA MARKERS
Abstract
The population genetic structure of Labeo calbasu collected from four wild and a hatchery population was studied using microsatellite DNA marker analysis. Five heterologous microsatellite markers (Lr10, Lr21, Lr24, Lr26 and CcatG1) developed from rohu and catla were analyzed to test the genetic variability of L. calbasu stocks. The number of alleles observed in the loci ranged from 2-10. The loci were found to be polymorphic (<P95) in all the populations. The average numbers of possessed alleles were higher by the four wild stocks than the hatchery stock. The average number of allele was the highest in the Jamuna population (5.8) and the least in the Hatchery population (4.8). The observed average heterozygosity (Ho) in the Jamuna population (0.776) was the highest followed by the Halda (0.667), the Haor (0.661) and the Padma (0.642) populations. Except loci Lr10 and Lr24 in the Halda and locus Lr10 in the Padma and Hatchery populations, significant deviations from Hardy-Weinberg Equilibrium (HWE) were detected in all cases. The FST values and the Nm values indicated high level of differentiation and a low level of gene flow between the populations. The largest genetic distance value (D = 0.543) was measured between the Jamuna and the Hatchery populations while the least value (D =0.124) was observed between the Padma and the Halda populations. The estimated genetic population structure and potential applications of microsatellite markers may assist the proper management of L. calbasu populations in the wild.
