Express Method for Isolation of Ready-to-Use 3D Chitin Scaffolds from <i>Aplysina archeri</i> (Aplysineidae: Verongiida) Demosponge
Christine Klinger,
Sonia Żółtowska-Aksamitowska,
Marcin Wysokowski,
Mikhail V. Tsurkan,
Roberta Galli,
Iaroslav Petrenko,
Tomasz Machałowski,
Alexander Ereskovsky,
Rajko Martinović,
Lyubov Muzychka,
Oleg B. Smolii,
Nicole Bechmann,
Viatcheslav Ivanenko,
Peter J. Schupp,
Teofil Jesionowski,
Marco Giovine,
Yvonne Joseph,
Stefan R. Bornstein,
Alona Voronkina,
Hermann Ehrlich
Affiliations
Christine Klinger
Institute of Physical Chemistry, TU Bergakademie-Freiberg, Leipziger str. 29, 09559 Freiberg, Germany
Sonia Żółtowska-Aksamitowska
Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 61131 Poznan, Poland
Marcin Wysokowski
Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 61131 Poznan, Poland
Mikhail V. Tsurkan
Leibnitz Institute of Polymer Research Dresden, 01069 Dresden, Germany
Roberta Galli
Clinical Sensoring and Monitoring, Department of Anesthesiology and Intensive Care Medicine, Faculty of Medicine, Technische Universität Dresden, 01307 Dresden, Germany
Iaroslav Petrenko
Institute of Electronics and Sensor Materials, TU Bergakademie Freiberg, Gustav Zeuner Str. 3, 09599 Freiberg, Germany
Tomasz Machałowski
Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 61131 Poznan, Poland
Alexander Ereskovsky
Institut Méditerranéen de Biodiversité et d’Ecologie (IMBE), CNRS, IRD, Aix Marseille Université, Avignon Université, Station Marine d’Endoume, 13003 Marseille, France
Rajko Martinović
Institute of Marine Biology, University of Montenegro, 85330 Kotor, Montenegro
Lyubov Muzychka
Institute of Bioorganic Chemistry and Petrochemistry, National Academy of Science of Ukraine, Murmanska Str., 1, 02094 Kyiv, Ukraine
Oleg B. Smolii
Institute of Bioorganic Chemistry and Petrochemistry, National Academy of Science of Ukraine, Murmanska Str., 1, 02094 Kyiv, Ukraine
Nicole Bechmann
Institute of Clinical Chemistry and Laboratory Medicine, University Hospital Carl Gustav Carus, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany
Viatcheslav Ivanenko
Department of Invertebrate Zoology, Biological Faculty, Lomonosov Moscow State University, 119992 Moscow, Russia
Peter J. Schupp
Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, Carl-von-Ossietzky-Straße 9-11, 26111 Oldenburg, Germany
Teofil Jesionowski
Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 61131 Poznan, Poland
Marco Giovine
Department of Sciences of Earth, Environment and Life, University of Genoa, Corso Europa 26, 16132 Genova, Italy
Yvonne Joseph
Institute of Electronics and Sensor Materials, TU Bergakademie Freiberg, Gustav Zeuner Str. 3, 09599 Freiberg, Germany
Stefan R. Bornstein
Department of Internal Medicine III, University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany
Alona Voronkina
National Pirogov Memorial Medical University, Vinnytsya, Department of Pharmacy, Pirogov str. 56, 21018, Vinnytsia, Ukraine
Hermann Ehrlich
Institute of Electronics and Sensor Materials, TU Bergakademie Freiberg, Gustav Zeuner Str. 3, 09599 Freiberg, Germany
Sponges are a valuable source of natural compounds and biomaterials for many biotechnological applications. Marine sponges belonging to the order Verongiida are known to contain both chitin and biologically active bromotyrosines. Aplysina archeri (Aplysineidae: Verongiida) is well known to contain bromotyrosines with relevant bioactivity against human and animal diseases. The aim of this study was to develop an express method for the production of naturally prefabricated 3D chitin and bromotyrosine-containing extracts simultaneously. This new method is based on microwave irradiation (MWI) together with stepwise treatment using 1% sodium hydroxide, 20% acetic acid, and 30% hydrogen peroxide. This approach, which takes up to 1 h, made it possible to isolate chitin from the tube-like skeleton of A. archeri and to demonstrate the presence of this biopolymer in this sponge for the first time. Additionally, this procedure does not deacetylate chitin to chitosan and enables the recovery of ready-to-use 3D chitin scaffolds without destruction of the unique tube-like fibrous interconnected structure of the isolated biomaterial. Furthermore, these mechanically stressed fibers still have the capacity for saturation with water, methylene blue dye, crude oil, and blood, which is necessary for the application of such renewable 3D chitinous centimeter-sized scaffolds in diverse technological and biomedical fields.
