Frontiers in Veterinary Science (Nov 2021)
A Novel Fiber-1-Edited and Highly Attenuated Recombinant Serotype 4 Fowl Adenovirus Confers Efficient Protection Against Lethal Challenge
- Yaru Mu,
- Yaru Mu,
- Yaru Mu,
- Yaru Mu,
- Quan Xie,
- Quan Xie,
- Quan Xie,
- Quan Xie,
- Weikang Wang,
- Weikang Wang,
- Weikang Wang,
- Weikang Wang,
- Hao Lu,
- Hao Lu,
- Hao Lu,
- Hao Lu,
- Mingjun Lian,
- Mingjun Lian,
- Mingjun Lian,
- Mingjun Lian,
- Wei Gao,
- Wei Gao,
- Wei Gao,
- Wei Gao,
- Tuofan Li,
- Tuofan Li,
- Tuofan Li,
- Tuofan Li,
- Zhimin Wan,
- Zhimin Wan,
- Zhimin Wan,
- Zhimin Wan,
- Hongxia Shao,
- Hongxia Shao,
- Hongxia Shao,
- Hongxia Shao,
- Aijian Qin,
- Aijian Qin,
- Aijian Qin,
- Aijian Qin,
- Jianqiang Ye,
- Jianqiang Ye,
- Jianqiang Ye,
- Jianqiang Ye
Affiliations
- Yaru Mu
- Ministry of Education Key Laboratory for Avian Preventive Medicine, Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, China
- Yaru Mu
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Yaru Mu
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China
- Yaru Mu
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Quan Xie
- Ministry of Education Key Laboratory for Avian Preventive Medicine, Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, China
- Quan Xie
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Quan Xie
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China
- Quan Xie
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Weikang Wang
- Ministry of Education Key Laboratory for Avian Preventive Medicine, Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, China
- Weikang Wang
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Weikang Wang
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China
- Weikang Wang
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Hao Lu
- Ministry of Education Key Laboratory for Avian Preventive Medicine, Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, China
- Hao Lu
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Hao Lu
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China
- Hao Lu
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Mingjun Lian
- Ministry of Education Key Laboratory for Avian Preventive Medicine, Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, China
- Mingjun Lian
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Mingjun Lian
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China
- Mingjun Lian
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Wei Gao
- Ministry of Education Key Laboratory for Avian Preventive Medicine, Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, China
- Wei Gao
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Wei Gao
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China
- Wei Gao
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Tuofan Li
- Ministry of Education Key Laboratory for Avian Preventive Medicine, Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, China
- Tuofan Li
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Tuofan Li
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China
- Tuofan Li
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Zhimin Wan
- Ministry of Education Key Laboratory for Avian Preventive Medicine, Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, China
- Zhimin Wan
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Zhimin Wan
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China
- Zhimin Wan
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Hongxia Shao
- Ministry of Education Key Laboratory for Avian Preventive Medicine, Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, China
- Hongxia Shao
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Hongxia Shao
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China
- Hongxia Shao
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Aijian Qin
- Ministry of Education Key Laboratory for Avian Preventive Medicine, Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, China
- Aijian Qin
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Aijian Qin
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China
- Aijian Qin
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, China
- Jianqiang Ye
- Ministry of Education Key Laboratory for Avian Preventive Medicine, Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, China
- Jianqiang Ye
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Jianqiang Ye
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China
- Jianqiang Ye
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, China
- DOI
- https://doi.org/10.3389/fvets.2021.759418
- Journal volume & issue
-
Vol. 8
Abstract
Currently, a fatal disease of hepatitis-hydropericardium syndrome (HHS) caused by serotype 4 fowl adenovirus (FAdV-4) has spread worldwide and resulted in tremendous economic losses to the poultry industry. Various vaccines against FAdV-4 were developed to control the disease; however, few live-attenuated vaccines were available. In this study, we targeted the N-terminal of fiber-1 and rescued a recombinant virus FAdV4-RFP_F1 expressing the fusion protein of RFP and Fiber-1 based on the CRISPR/Cas9 technique. In vitro studies showed that FAdV4-RFP_F1 replicated slower than the wild type FAdV-4, but the peak viral titer of FAdV4-RFP_F1 could still reach 107.0 TCID50/ml with high stability in LMH cells. Animal studies found that FAdV4-RFP_F1 not only was highly attenuated to the 2-week-old SPF chickens, but could also provide efficient protection against lethal challenge of FAdV-4. All these demonstrate that the recombinant virus FAdV4-RFP_F1 could be as an efficient live-attenuated vaccine candidate for FAdV-4, and the N-terminal of fiber-1 could be as a potential insertion site for expressing foreign genes to develop FAdV-4-based vaccine.
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