Nature Communications (Oct 2020)
Analysis of protein-DNA interactions in chromatin by UV induced cross-linking and mass spectrometry
Abstract
Cross-linking mass spectrometry (XLMS) allows mapping of protein-protein and protein-RNA interactions, but the analysis of protein-DNA complexes remains challenging. Here, the authors develop a UV light-based XLMS workflow to determine protein-DNA interfaces in reconstituted chromatin and isolated nuclei.