Extra Virgin Olive Oil Extracts Modulate the Inflammatory Ability of Murine Dendritic Cells Based on Their Polyphenols Pattern: Correlation between Chemical Composition and Biological Function
Stefania De Santis,
Marina Liso,
Giulio Verna,
Francesca Curci,
Gualtiero Milani,
Maria Felicia Faienza,
Carlo Franchini,
Antonio Moschetta,
Marcello Chieppa,
Maria Lisa Clodoveo,
Pasquale Crupi,
Filomena Corbo
Affiliations
Stefania De Santis
Department of Pharmacy-Drug Science, University of Bari Aldo Moro, 70126 Bari, Italy
Marina Liso
National Institute of Gastroenterology “S. de Bellis”, Research Hospital, 70013 Castellana Grotte, Italy
Giulio Verna
Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy
Francesca Curci
Department of Pharmacy-Drug Science, University of Bari Aldo Moro, 70126 Bari, Italy
Gualtiero Milani
Department of Pharmacy-Drug Science, University of Bari Aldo Moro, 70126 Bari, Italy
Maria Felicia Faienza
Pediatric Unit, Department of Biomedical Sciences and Human Oncology, University of Bari Aldo Moro, 70124 Bari, Italy
Carlo Franchini
Department of Pharmacy-Drug Science, University of Bari Aldo Moro, 70126 Bari, Italy
Antonio Moschetta
Department of Interdisciplinary Medicine, University of Bari Aldo Moro, 70124 Bari, Italy
Marcello Chieppa
National Institute of Gastroenterology “S. de Bellis”, Research Hospital, 70013 Castellana Grotte, Italy
Maria Lisa Clodoveo
Department of Interdisciplinary Medicine, University of Bari Aldo Moro, 70124 Bari, Italy
Pasquale Crupi
Department of Interdisciplinary Medicine, University of Bari Aldo Moro, 70124 Bari, Italy
Filomena Corbo
Department of Pharmacy-Drug Science, University of Bari Aldo Moro, 70126 Bari, Italy
Extra virgin olive oil (EVOO) represents one of the most important health-promoting foods whose antioxidant and anti-inflammatory activities are mainly associated to its polyphenols content. To date, studies exploring the effect of EVOO polyphenols on dendritic cells (DCs), acting as a crosstalk between the innate and the adaptive immune response, are scanty. Therefore, we studied the ability of three EVOO extracts (cv. Coratina, Cima di Mola/Coratina, and Casaliva), characterized by different polyphenols amount, to regulate DCs maturation in resting conditions or after an inflammatory stimulus. Cima di Mola/Coratina and Casaliva extracts were demonstrated to be the most effective in modulating DCs toward an anti-inflammatory profile by reduction of TNF and IL-6 secretion and CD86 expression, along with a down-modulation of Il-1β and iNOS expression. From factorial analysis results, 9 polyphenols were tentatively established to play a synergistic role in modulating DCs inflammatory ability, thus reducing the risk of chronic inflammation.
