Advanced Science (Apr 2024)
Large‐Scale Proteome Profiling Identifies Biomarkers Associated with Suspected Neurosyphilis Diagnosis
- Jun Li,
- Jie Ma,
- MingJuan Liu,
- Mansheng Li,
- Ming Zhang,
- Wenhao Yin,
- Mengyin Wu,
- Xiao Li,
- Qiyu Zhang,
- Hanlin Zhang,
- Heyi Zheng,
- Chenhui Mao,
- Jian Sun,
- Wenze Wang,
- Wei Lyu,
- Xueping Yue,
- Wenjia Weng,
- Juan Li,
- Fengxin Chen,
- Yunping Zhu,
- Ling Leng
Affiliations
- Jun Li
- Department of Dermatology Institute of Clinical Medicine State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College National Clinical Research Center for Dermatologic and Immunologic Diseases Beijing 100730 China
- Jie Ma
- State Key Laboratory of Medical Proteomics Beijing Proteome Research Center National Center for Protein Sciences (Beijing) Beijing Institute of Lifeomics Beijing 102206 China
- MingJuan Liu
- Department of Dermatology Institute of Clinical Medicine State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College National Clinical Research Center for Dermatologic and Immunologic Diseases Beijing 100730 China
- Mansheng Li
- State Key Laboratory of Medical Proteomics Beijing Proteome Research Center National Center for Protein Sciences (Beijing) Beijing Institute of Lifeomics Beijing 102206 China
- Ming Zhang
- Department of Dermatology Beijing Youan Hospital Capital Medical University Beijing 100069 China
- Wenhao Yin
- The First Hospital of Jiaxing The Affiliated Hospital of Jiaxing University Zhejiang 314001 China
- Mengyin Wu
- Department of Dermatology Institute of Clinical Medicine State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College National Clinical Research Center for Dermatologic and Immunologic Diseases Beijing 100730 China
- Xiao Li
- State Key Laboratory of Medical Proteomics Beijing Proteome Research Center National Center for Protein Sciences (Beijing) Beijing Institute of Lifeomics Beijing 102206 China
- Qiyu Zhang
- Department of Dermatology Institute of Clinical Medicine State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College National Clinical Research Center for Dermatologic and Immunologic Diseases Beijing 100730 China
- Hanlin Zhang
- Department of Dermatology Institute of Clinical Medicine State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College National Clinical Research Center for Dermatologic and Immunologic Diseases Beijing 100730 China
- Heyi Zheng
- Department of Dermatology Institute of Clinical Medicine State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College National Clinical Research Center for Dermatologic and Immunologic Diseases Beijing 100730 China
- Chenhui Mao
- Department of Neurology Peking Union Medical College Hospital Peking Union Medical College and Chinese Academy of Medical Science Beijing 100730 China
- Jian Sun
- Department of Pathology Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100730 China
- Wenze Wang
- Department of Pathology Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100730 China
- Wei Lyu
- Department of Infectious Disease Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100730 China
- Xueping Yue
- Department of Dermatology and Venereology Beijing Tiantan Hospital Capital Medical University Beijing 100050 China
- Wenjia Weng
- Department of Dermatology Beijing Youan Hospital Capital Medical University Beijing 100069 China
- Juan Li
- Department of Dermatology Beijing Youan Hospital Capital Medical University Beijing 100069 China
- Fengxin Chen
- Infections Disease Center Beijing Ditan Hospital Capital Medical University Beijing 100102 China
- Yunping Zhu
- State Key Laboratory of Medical Proteomics Beijing Proteome Research Center National Center for Protein Sciences (Beijing) Beijing Institute of Lifeomics Beijing 102206 China
- Ling Leng
- Stem cell and Regenerative Medicine Lab Department of Medical Science Research Center Institute of Clinical Medicine State Key Laboratory of Complex Severe and Rare Diseases Translational Medicine Center Peking Union Medical College Hospital Peking Union Medical College and Chinese Academy of Medical Sciences Beijing 100730 China
- DOI
- https://doi.org/10.1002/advs.202307744
- Journal volume & issue
-
Vol. 11,
no. 16
pp. n/a – n/a
Abstract
Abstract Neurosyphilis (NS) is a central nervous system (CNS) infection caused by Treponema pallidum (T. pallidum). NS can occur at any stage of syphilis and manifests as a broad spectrum of clinical symptoms. Often referred to as “the great imitator,” NS can be easily overlooked or misdiagnosed due to the absence of standard diagnostic tests, potentially leading to severe and irreversible organ dysfunction. In this study, proteomic and machine learning model techniques are used to characterize 223 cerebrospinal fluid (CSF) samples to identify diagnostic markers of NS and provide insights into the underlying mechanisms of the associated inflammatory responses. Three biomarkers (SEMA7A, SERPINA3, and ITIH4) are validated as contributors to NS diagnosis through multicenter verification of an additional 115 CSF samples. We anticipate that the identified biomarkers will become effective tools for assisting in diagnosis of NS. Our insights into NS pathogenesis in brain tissue may inform therapeutic strategies and drug discoveries for NS patients.
Keywords
