Spatial regulation of AMPK signaling revealed by a sensitive kinase activity reporter

Danielle L. Schmitt; Stephanie D. Curtis; Anne C. Lyons; Jin-fan Zhang; Mingyuan Chen; Catherine Y. He; Sohum Mehta; Reuben J. Shaw; Jin Zhang

doi:10.1038/s41467-022-31190-x

Nature Communications (Jul 2022)

Spatial regulation of AMPK signaling revealed by a sensitive kinase activity reporter

Danielle L. Schmitt,
Stephanie D. Curtis,
Anne C. Lyons,
Jin-fan Zhang,
Mingyuan Chen,
Catherine Y. He,
Sohum Mehta,
Reuben J. Shaw,
Jin Zhang

Affiliations

Danielle L. Schmitt: Department of Pharmacology, University of California San Diego
Stephanie D. Curtis: Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies
Anne C. Lyons: Department of Bioengineering, University of California San Diego
Jin-fan Zhang: Department of Bioengineering, University of California San Diego
Mingyuan Chen: Department of Bioengineering, University of California San Diego
Catherine Y. He: Department of Pharmacology, University of California San Diego
Sohum Mehta: Department of Pharmacology, University of California San Diego
Reuben J. Shaw: Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies
Jin Zhang: Department of Pharmacology, University of California San Diego

DOI: https://doi.org/10.1038/s41467-022-31190-x
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 12

Abstract

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AMP activated protein kinase (AMPK) is a master regulator of cellular metabolism, but how AMPK activity is spatiotemporally regulated remains unclear. Here, Schmitt et al develop a sensitive biosensor for AMPK, which they use to uncover mechanisms for AMPK activity in the lysosome and nucleus.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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