Iranian Journal of Public Health (Aug 2007)

Detection of Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyticum by Multiplex PCR in Semen Sample of Infertile Men

  • M Golshani,
  • G Eslami,
  • Sh Mohhammadzadeh Ghobadloo,
  • F Fallah,
  • H Goudarzi,
  • AA Soleimani Rahbar,
  • F Fayaz

Journal volume & issue
Vol. 36, no. 2
pp. 50 – 57

Abstract

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Background: The aim of this study was to detect Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyti¬cum from semen samples of infertile men by Multiplex PCR and investigation of influence of bacteriospermia on semen parame¬ters. Methods: Semen samples of 200 infertile men were evaluated by Multiplex PCR. In addition, analysis of semen parameters was performed according to the WHO guidelines. Results: All the patients were without clinical symptoms of urogenital tract infection. Thirty three percent of cases showed at least one bacterium. We found a noticeable relation between the presence of bacteriospermia and the rate of non motile and morphologically abnormal sperms (P 0.05). Conclusion: Asymptomatic existence of Chlamydia and Mycoplasmas in urogenital tracts might play an important role in sperm impairment due to infertility. Bacteriospermia can influence sperm's motility, morphology and concentration.

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