Expression of CD25+ and CD95+ Surface Markers on Peripheral Blood Lymphocytes in Patients with Vibration Disease

Abstract

Vibration disease occupies one of the leading places among occupational diseases. The development and course of vibration disease is accompanied by disorders in immune system.Aim of the study is to provide a comparative assessment of cells with markers of the early (CD25+) and late (CD95+) stage of lymphocyte activation and to compare them with the phenotypic composition of lymphocytes in patients with vibration disease.Materials and methods. The study includes men aged 36-60 years with vibration disease from exposure to local vibration (26 people) and from combined exposure to local and general vibration (28 people).Results. The most pronounced changes in the system of lymphocyte apoptosis are observed in patients with vibration disease from the combined effects of local and general vibration, manifested by the increase in the number of CD25+ and CD95+-lymphocytes. Increase of quantity of CD25+ at patients with a vibration disease from impact of local vibration and from the combined impact of local and general vibration is caused by increase in their number on lymphocytes what the direct correlation dependence between quantity of CD25+-lymphocytes and the number of CD3+, CD4+, CD8+, CD16+, CD20+-lymphocytes testifies to.Conclusions. In patients with vibration disease from the combined effects of local and general vibration, the increase in the number of CD95+ is due to an increase in their density on mature T-lymphocytes, as evidenced by the inverse relationship between the level of CD95+ and the CD3+-lymphocytes. The obtained data should be taken into account in order to develop new effective methods of prevention, treatment and prediction of the course of vibration pathology.

Keywords

• vibration disease
• exposure to local and combined vibration
• lymphocyte populations and subpopulations